Covalent Modification of Phosphatidylethanolamine by Benzyl Isothiocyanate and the Resultant Generation of Ethanolamine Adduct as Its Metabolite
| Autor: | Toshiyuki Nakamura, Noritoshi Kitamoto, Akari Ishisaka, Miho Hirakawa, Akira Murakami, Yoshimasa Nakamura, Yoji Kato |
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| Rok vydání: | 2019 |
| Předmět: |
Phosphatidylethanolamine
Molecular Structure Benzyl isothiocyanate Stereochemistry Phosphatidylethanolamines General Medicine Phosphatidic acid Toxicology Lipoprotein particle Adduct Mice chemistry.chemical_compound RAW 264.7 Cells Ethanolamine chemistry Isothiocyanates Liposomes Isothiocyanate Animals Moiety |
| Zdroj: | Chemical Research in Toxicology. 32:638-644 |
| ISSN: | 1520-5010 0893-228X |
| DOI: | 10.1021/acs.chemrestox.8b00331 |
| Popis: | Benzyl isothiocyanate (BITC), a dietary isothiocyanate (ITC) derived from cruciferous vegetables, has anticancer properties. It is believed that the ITC moiety (-N═C═S) that reacts predominantly with thiol compounds plays a central role in triggering the activities resulting from these properties. Recent studies have demonstrated that ITCs also covalently modify amino moieties in a protein. In this study, we examined the chemical reaction between BITC and the aminophospholipid, phosphatidylethanolamine (PE), in the cell membrane or lipoprotein particle. To detect the BITC-modified PE, the bond between ethanolamine (EA) and phosphatidic acid in PE was cleaved using phospholipase D to form the BITC-EA adduct, which was then measured. BITC-EA was detected from the BITC-treated unilamellar liposome and low-density lipoprotein even with only a few micromoles of BITC treatment, suggesting that BITC might react with not only a thiol/amino group of a protein but also an amino moiety of an aminophospholipid. Moreover, after incorporating BITC-PE included in the liposomes into the cultured cells or after direct exposure of BITC to the cells, free BITC-EA was excreted and accumulated in the medium in a time-dependent manner. It indicates that an intracellular enzyme catalyzes the cleavage of BITC-PE to produce BITC-EA. Because the ITC-amine adduct is stable, the ITC-EA adduct could be a promising indicator of ITC exposure in vivo. |
| Databáze: | OpenAIRE |
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