Characterisation of Lactobacillus helveticus strains producing antihypertensive peptides by RAPD and inverse-PCR of IS elements
Autor: | A Carbini, R Strappati, Gloria Silvestri, Lucia Aquilanti, Cristiana Garofalo, Sabrina Santarelli, Francesca Clementi |
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Rok vydání: | 2010 |
Předmět: |
Microbiology (medical)
Lactobacillus helveticus Inverse polymerase chain reaction Molecular Sequence Data food and beverages Biology biology.organism_classification Polymerase Chain Reaction Microbiology Random Amplified Polymorphic DNA Technique RAPD law.invention Probiotic DNA profiling law DNA Transposable Elements Multiplex Food science Insertion sequence Peptides Antihypertensive Agents Phylogeny Polymerase chain reaction |
Zdroj: | Beneficial Microbes. 1:229-242 |
ISSN: | 1876-2891 1876-2883 |
Popis: | Lactobacillus helveticus is used for the manufacture of cheeses and milk-based products. Although it is not considered a probiotic microorganism, some strains demonstrated beneficial effects through the production of antihypertensive peptides from the hydrolysis of casein during milk fermentation. Strain-specificity of bioactive peptide production by L. helveticus makes the availability of reliable typing methods essential for both legal and good manufacturing processes. Accordingly, RAPD and inverse-PCR of five insertion sequence elements were comparatively evaluated for the molecular characterisation of four L. helveticus dairy cultures producing antihypertensive peptides and fourteen reference strains. Calculation of discriminatory indices and cluster analysis of the DNA fingerprints confirmed the suitability of both approaches for acceptable strain differentiation. Although RAPD was more discriminating, for a few test strains a neat discrimination was only achieved through multiplex inverse-PCR, thus suggesting the suitability of a combined analytical approach for a finer strain discrimination. |
Databáze: | OpenAIRE |
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