In vitro studies on the potential use of 5-aminolaevulinic acid-mediated photodynamic therapy for gynaecological tumours
| Autor: | Eva F. Gudgin Dickson, D. L. Campbell, Francesca Rossi, James C. Kennedy, Roy H. Pottier |
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| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Genital Neoplasms Female medicine.medical_treatment Drug Resistance Protoporphyrins Photodynamic therapy Biology chemistry.chemical_compound Ovarian carcinoma Carcinoma medicine Fluorescence microscope polycyclic compounds Humans Photosensitizer Protoporphyrin IX Aminolevulinic Acid medicine.disease In vitro Oncology chemistry Photochemotherapy Cancer research Protoporphyrin Female Research Article HeLa Cells |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Results are reported on the sensitivity of various gynaecological tumour cell lines to 5-aminolaevulinic acid-induced protoporphyrin IX-sensitised photodynamic therapy (ALA-PDT) in vitro. All cell lines tested accumulated ALA-induced protoporphyrin IX (PpIX) and demonstrated good sensitivity to ALA-PDT. Localisation of PpIX in the mitochondria was demonstrated by fluorescence microscopy. Subcellular damage following ALA-PDT was observed using transmission electron microscopy. This damage was localised initially to the mitochondria, with damage to membranes and the nucleus and complete loss of intracytoplasmic organisation being observed subsequently. There was no apparent difference in ALA-PDT response between a multidrug-resistant ovarian carcinoma cell line and its parent line. These results indicate that ALA-PDT has potential for application to therapy of gynaecological malignancies. Images Figure 5 Figure 6 Figure 7 |
| Databáze: | OpenAIRE |
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