Evaluation of the Antimicrobial Potential and Toxicity of a Newly Synthesised 4-(4-(Benzylamino)butoxy)-9H-carbazole
| Autor: | Katarzyna Lisowska, Dorota G. Piotrowska, Aleksandra Felczak, Iwona E. Głowacka, Katarzyna Zawadzka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Gram-negative bacteria
antibacterial agent QH301-705.5 Gram-positive bacteria Carbazoles Aspergillus flavus Bacterial growth Catalysis Article Inorganic Chemistry chemistry.chemical_compound Anti-Infective Agents Yeasts Humans Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy Antibacterial agent antimicrobial activity biology Bacteria Carbazole carbazole derivative antifungal properties Organic Chemistry Fungi General Medicine Fibroblasts Antimicrobial biology.organism_classification Haemolysis Computer Science Applications Chemistry chemistry Nuclear chemistry |
| Zdroj: | International Journal of Molecular Sciences; Volume 22; Issue 23; Pages: 12796 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 12796, p 12796 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms222312796 |
| Popis: | One of the greatest threats to human and animal health is posed by infections caused by drug-resistant bacterial strains. Therefore, newly synthesised substances are tested for their antimicrobial activity. Carbazole derivatives seem to be promising antibacterial agents. This study aimed at investigating the toxicity and activity of newly synthesised, functionalised carbazole derivative 2 (4-(4-(benzylamino)butoxy)-9H-carbazole) against various microorganisms. Its antimicrobial potential against Gram-positive and Gram-negative bacteria, yeast, and filamentous fungi was examined according to CLSI (Clinical and Laboratory Standards Institute) standards. The tested compound was found to efficiently inhibit the growth of Gram-positive strains. The addition of carbazole derivative 2 at the concentration of 30 µg/mL caused inhibition of bacterial growth by over 95%. Moreover, about 50 and 45% limitation of Pseudomonas aeruginosa and Aspergillus flavus growth was observed in the samples incubated with the addition of 20 and 60 µg/mL of the compound, respectively. Its addition to the microbial cultures caused an increase in the permeability of the cellular membrane. Slight haemolysis of red blood cells was observed after 24-h treatment with carbazole derivative 2. On the other hand, human fibroblasts were found to be more sensitive to its effects. The activity of the tested compound indicates a possibility of its further modification in order to obtain effective drugs, especially against drug-resistant staphylococci. |
| Databáze: | OpenAIRE |
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