Metformin associated with photodynamic therapy – A novel oncological direction
| Autor: | Lucian Craciun, Corina Tatomir, Adriana Muresan, Diana Olteanu, Mihaela Aldea, Iuliana Nenu, Rodica M. Ion, Tiberiu Popescu, Pompei Bolfa, Adriana Filip |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Radiation-Sensitizing Agents Porphyrins Necrosis medicine.medical_treatment Biophysics Nitric Oxide Synthase Type II Apoptosis Photodynamic therapy Pharmacology Nitric Oxide chemistry.chemical_compound In vivo Cell Line Tumor Malondialdehyde Neoplasms Radiation Ionizing medicine Animals Hypoglycemic Agents Radiology Nuclear Medicine and imaging Rats Wistar Radiation Radiological and Ultrasound Technology biology business.industry Nitrotyrosine Glutathione Metformin Rats Nitric oxide synthase Matrix Metalloproteinase 9 Photochemotherapy chemistry Cyclooxygenase 2 biology.protein Matrix Metalloproteinase 2 medicine.symptom business medicine.drug |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 138:80-91 |
| ISSN: | 1011-1344 |
| Popis: | The aim of our study was to assess the effect of the combined treatment of Metformin (Metf) and 5, 10, 15, 20-tetra-sulfophenyl-porphyrin (TSPP)–mediated photodynamic therapy (PDT) on an in vivo tumour model. Wistar male rats were divided in 6 groups: group 1, treated with TSPP; groups 2 and 4 treated with TSPP and Metf, respectively, and irradiated 24 h thereafter; group 3 was treated with Metf and the last two groups received the combined treatment, Metf administered prior (group 5) or after (group 6) irradiation. 72 h from the start of the treatment, tumour tissue was sampled for the investigation of oxidative and nitrosative stress. The apoptotic rate, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expressions and matrix metalloproteinases activities were also quantified. Malondialdehyde and glutathione levels were significantly elevated in the groups treated with combined therapy (p < 0.05). Metf associated with TSPP–PDT reduced iNOS and COX-2 expressions and enhanced nitrotyrosine levels in both therapeutic regimens. Peroxynitrate formation and its cytotoxic effect on tumour cells were related to an elevated index of apoptosis and necrosis. Moreover, MMP-2 activity reached a minimum in the groups which received combined therapy. Our results confirmed that the association of Metf with PDT might prove a new and promising oncological approach. |
| Databáze: | OpenAIRE |
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