Synthesis of DNA Complements of Natural RNAs: A General Approach
| Autor: | Sol Spiegelman, D. L. Kacian, K. F. Watson |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
DNA polymerase
Tritium Nucleic acid thermodynamics chemistry.chemical_compound Complementary DNA Thymine Nucleotides Bacteriophages Gene Genetics Multidisciplinary Avian Leukosis Virus biology Nucleic Acid Hybridization RNA Complement System Proteins Templates Genetic Reverse transcriptase RNA Bacterial chemistry DNA Nucleotidyltransferases DNA Viral biology.protein RNA Viral Biological Sciences: Biochemistry Moloney murine leukemia virus Oncovirus DNA |
| Zdroj: | Proceedings of the National Academy of Sciences. 68:2843-2845 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.68.11.2843 |
| Popis: | The availability of a purified RNA-instructed DNA polymerase (reverse transcriptase) from avain myeloblastosis virus provided the opportunity to explore whether this enzyme could be used as a general tool for synthesizing DNA complements of a wide variety of natural RNAs. The results described show that this potentially useful situation is in fact realized. The avian viral transcriptase can mediate the synthesis of DNA complementary to RNAs of such widely divergent origins as Qβ bacteriophage and Moloney sarcoma virus. These findings open up novel pathways for the experimental resolution of several interesting problems. Thus, given a purified RNA message, one should be able to synthesize the corresponding DNA genetic material. If suitably labeled, the synthetic DNA has various obvious uses, including its use via molecular hybridization as an analytical probe for the corresponding gene on the chromosomes or for its message in a complex mixture of RNA molecules. Of immediate practical interest is the import of these findings for viral oncology. They imply that for many purposes we will not be compelled to isolate or use the “reverse transcriptase” from each oncogenic virus in order to synthesize its complementary DNA. The ability of one enzyme to accept a variety of oncogenic RNAs will obviate many of the logistical difficulties that arise, particularly in attempts to illuminate the etiology of human cancer. |
| Databáze: | OpenAIRE |
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