Lipids Are the Preferred Substrate of the Protist Naegleria gruberi, Relative of a Human Brain Pathogen
| Autor: | Bexkens, Michiel L, Zimorski, Verena, Sarink, Maarten J, Wienk, Hans, Brouwers, Jos F, De Jonckheere, Johan F, Martin, William F, Opperdoes, Fred R, van Hellemond, Jaap J, Tielens, Aloysius G M, Sub NMR Spectroscopy, LS Veterinaire biochemie, Sub MS-faciliteit, dB&C FR-RMSC RMSC, dB&C FR-RMSC FR, Dep Biochemie en Celbiologie |
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| Přispěvatelé: | Sub NMR Spectroscopy, LS Veterinaire biochemie, Sub MS-faciliteit, dB&C FR-RMSC RMSC, dB&C FR-RMSC FR, Dep Biochemie en Celbiologie, Medical Microbiology & Infectious Diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030106 microbiology Protozoan Proteins Respiratory chain medicine.disease_cause Naegleria Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences alternative oxidase 0302 clinical medicine Lipid oxidation parasitic diseases medicine Humans lcsh:QH301-705.5 Beta oxidation Pathogen Naegleria fowleri fatty acid oxidation aerobic energy metabolism 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Chemistry Naegleria gruberi Brain Protist Genomics Metabolism biology.organism_classification Lipids Amino acid Oxygen Glucose 030104 developmental biology lcsh:Biology (General) Biochemistry electron-transport chain PAM Genome Protozoan 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 25, Iss 3, Pp 537-543.e3 (2018) Cell Reports Cell Reports, 25(3), 537-+. Cell Press Cell Reports, 25(3), 537. Elsevier Saunders |
| ISSN: | 2211-1247 |
| Popis: | Summary Naegleria gruberi is a free-living non-pathogenic amoeboflagellate and relative of Naegleria fowleri, a deadly pathogen causing primary amoebic meningoencephalitis (PAM). A genomic analysis of N. gruberi exists, but physiological evidence for its core energy metabolism or in vivo growth substrates is lacking. Here, we show that N. gruberi trophozoites need oxygen for normal functioning and growth and that they shun both glucose and amino acids as growth substrates. Trophozoite growth depends mainly upon lipid oxidation via a mitochondrial branched respiratory chain, both ends of which require oxygen as final electron acceptor. Growing N. gruberi trophozoites thus have a strictly aerobic energy metabolism with a marked substrate preference for the oxidation of fatty acids. Analyses of N. fowleri genome data and comparison with those of N. gruberi indicate that N. fowleri has the same type of metabolism. Specialization to oxygen-dependent lipid breakdown represents an additional metabolic strategy in protists. Graphical Abstract Highlights • Naegleria gruberi is a strict aerobe and needs oxygen for normal functioning and growth • Unique among protists, N. gruberi prefers lipids over glucose as an energy source • Lipid breakdown proceeds via a branched respiratory chain, both ends using oxygen • N. fowleri, the fatal human brain amoeba, is predicted to have the same food preference Bexkens et al. show that N. gruberi amoebae live preferably on lipids, for which they need oxygen, a lifestyle largely unknown among protists. This challenges existing views about its energy metabolism, with implications for treatment of its pathogenic relative, N. fowleri, the brain-eating agent of primary amoebic meningoencephalitis (PAM). |
| Databáze: | OpenAIRE |
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