Salmonella maintains the integrity of its intracellular vacuole through the action of SifA
| Autor: | Steve Garvis, Timothy A. Ryder, Kate E. Unsworth, Scott R. Waterman, Javier Ruiz-Albert, Carmen R. Beuzón, David W. Holden, Emmanuel Boucrot, Stéphane Méresse |
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| Rok vydání: | 2000 |
| Předmět: |
Salmonella typhimurium
Recombinant Fusion Proteins Molecular Sequence Data Mutant Virulence Vacuole Biology General Biochemistry Genetics and Molecular Biology Cell Line Microbiology Type three secretion system Mice Bacterial Proteins Animals Humans Amino Acid Sequence Molecular Biology DNA Primers Salmonella Infections Animal Host cell cytosol Base Sequence Sequence Homology Amino Acid General Immunology and Microbiology General Neuroscience Articles Entry into host Pathogenicity island Genes Bacterial Vacuoles bacteria Intracellular HeLa Cells Protein Binding |
| Zdroj: | The EMBO Journal. 19:3235-3249 |
| ISSN: | 1460-2075 |
| DOI: | 10.1093/emboj/19.13.3235 |
| Popis: | A method based on the Competitive Index was used to identify Salmonella typhimurium virulence gene interactions during systemic infections of mice. Analysis of mixed infections involving single and double mutant strains showed that OmpR, the type III secretion system of Salmonella pathogenicity island 2 (SPI-2) and SifA [required for the formation in epithelial cells of lysosomal glycoprotein (lgp)-containing structures, termed Sifs] are all involved in the same virulence function. sifA gene expression was induced after Salmonella entry into host cells and was dependent on the SPI-2 regulator ssrA. A sifA(-) mutant strain had a replication defect in macrophages, similar to that of SPI-2 and ompR(-) mutant strains. Whereas wild-type and SPI-2 mutant strains reside in vacuoles that progressively acquire lgps and the vacuolar ATPase, the majority of sifA(-) bacteria lost their vacuolar membrane and were released into the host cell cytosol. We propose that the wild-type strain, through the action of SPI-2 effectors (including SpiC), diverts the Salmonella-containing vacuole from the endocytic pathway, and subsequent recruitment and maintenance of vacuolar ATPase/lgp-containing membranes that enclose replicating bacteria is mediated by translocation of SifA. |
| Databáze: | OpenAIRE |
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