CXCL13/CXCR5 Interaction Facilitates VCAM-1-Dependent Migration in Human Osteosarcoma
| Autor: | Chiang-Wen Lee, Chih-Yang Lin, Ju Fang Liu, Chih-Hsin Tang, Chia Chia Chao, Yuan Li Huang, Yi-Chin Fong, Tsung Ming Chang, Chien Kuo Han |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Receptors
CXCR5 Chemokine Vascular Cell Adhesion Molecule-1 Bone Neoplasms migration Catalysis CXCR5 Article Inorganic Chemistry lcsh:Chemistry chemistry.chemical_compound Cell Movement osteosarcoma Tumor Cells Cultured medicine Humans Neoplasm Invasiveness Physical and Theoretical Chemistry VCAM-1 CXCL13 Molecular Biology lcsh:QH301-705.5 Spectroscopy biology Cell adhesion molecule Organic Chemistry Cell migration General Medicine medicine.disease invasion Chemokine CXCL13 Computer Science Applications chemistry lcsh:Biology (General) lcsh:QD1-999 Cancer research biology.protein Osteosarcoma Signal transduction Protein Binding Signal Transduction |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 17 International Journal of Molecular Sciences, Vol 21, Iss 6095, p 6095 (2020) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms21176095 |
| Popis: | Osteosarcoma is the most common primary tumor of the skeletal system and is well-known to have an aggressive clinical outcome and high metastatic potential. The chemokine (C-X-C motif) ligand 13 (CXCL13) plays a vital role in the development of several cancers. However, the effect of CXCL13 in the motility of osteosarcoma cells remains uncertain. Here, we found that CXCL13 increases the migration and invasion potential of three osteosarcoma cell lines. In addition, CXCL13 expression was upregulated in migration-prone MG-63 cells. Vascular cell adhesion molecule 1 (VCAM-1) siRNA and antibody demonstrated that CXCL13 promotes migration via increasing VCAM-1 production. We also show that CXCR5 receptor controls CXCL13-mediated VCAM-1 expression and cell migration. Our study identified that CXCL13/CXCR5 axis facilitate VCAM-1 production and cell migration in human osteosarcoma via the phospholipase C beta (PLC&beta ), protein kinase C &alpha (PKC&alpha ), c-Src, and nuclear factor-&kappa B (NF-&kappa B) signaling pathways. CXCL13 and CXCR5 appear to be a novel therapeutic target in metastatic osteosarcoma. |
| Databáze: | OpenAIRE |
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