Isolation of Mitochondrial DNA from Single, Short Hairs without Roots Using Pressure Cycling Technology
| Autor: | Kathryn A. Harper, Kelly A. Meiklejohn, Constance L. Fisher, James M. Robertson, Richard T Merritt, Jessica Walker |
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| Rok vydání: | 2018 |
| Předmět: |
Automation
Laboratory Mitochondrial DNA Chromatography 010401 analytical chemistry Analytical chemistry Forensic Medicine Biology Isolation (microbiology) DNA Mitochondrial 01 natural sciences Specimen Handling 0104 chemical sciences Computer Science Applications Nuclear DNA 03 medical and health sciences Medical Laboratory Technology 0302 clinical medicine Hydrostatic Pressure Humans Pressure cycling 030216 legal & forensic medicine Hair |
| Zdroj: | SLAS Technology. 23:97-105 |
| ISSN: | 2472-6303 |
| DOI: | 10.1177/2472630317732073 |
| Popis: | Hairs are commonly submitted as evidence to forensic laboratories, but standard nuclear DNA analysis is not always possible. Mitochondria (mt) provide another source of genetic material; however, manual isolation is laborious. In a proof-of-concept study, we assessed pressure cycling technology (PCT; an automated approach that subjects samples to varying cycles of high and low pressure) for extracting mtDNA from single, short hairs without roots. Using three microscopically similar donors, we determined the ideal PCT conditions and compared those yields to those obtained using the traditional manual micro-tissue grinder method. Higher yields were recovered from grinder extracts, but yields from PCT extracts exceeded the requirements for forensic analysis, with the DNA quality confirmed through sequencing. Automated extraction of mtDNA from hairs without roots using PCT could be useful for forensic laboratories processing numerous samples. |
| Databáze: | OpenAIRE |
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