Multi-dimensional flow cytometry analysis reveals increasing changes in the systemic neutrophil compartment during seven consecutive days of endurance exercise

Autor:	Nienke Vrisekoop, Gerjen H. Tinnevelt, Leo Koenderman, Twan ten Haaf, Margot Klöpping, Selma van Staveren, Jos J. de Koning, Jeroen J. Jansen, Romain Meeusen, Bart Roelands, Bart Hilvering, Maria Francesca Piacentini, Karin de Ruiter
Přispěvatelé:	Physiotherapy, Human Physiology and Anatomy, Human Physiology and Sports Physiotherapy Research Group, Advanced Rehabilitation Technology & Science, Spine Research Group, Pulmonary medicine, Physiology, AMS - Sports and Work
Rok vydání:	2018
Předmět:	0301 basic medicine Male Neutrophils Physiology lcsh:Medicine Integrin alpha4beta1 Pathology and Laboratory Medicine Biochemistry Cortisol Analytical Chemistry White Blood Cells 0302 clinical medicine Spectrum Analysis Techniques Animal Cells Blood plasma Medicine and Health Sciences Public and Occupational Health Lipid Hormones L-Selectin Receptors Immunologic lcsh:Science Immune Response Multidisciplinary CD11b Antigen biology medicine.diagnostic_test Agricultural and Biological Sciences(all) Degranulation Compartment (chemistry) Middle Aged Flow Cytometry Healthy Volunteers Sports Science Body Fluids Phenotype Blood Integrin alpha M Spectrophotometry Female Cytophotometry medicine.symptom Cellular Types Anatomy Research Article Adult medicine.medical_specialty Immune Cells Immunology Inflammation GPI-Linked Proteins Research and Analysis Methods Blood Plasma Flow cytometry 03 medical and health sciences Signs and Symptoms Endurance training Diagnostic Medicine Internal medicine medicine Humans Sports and Exercise Medicine Exercise Steroid Hormones Blood Cells business.industry Biochemistry Genetics and Molecular Biology(all) lcsh:R Receptors IgG Biology and Life Sciences 030229 sport sciences Cell Biology Physical Activity Hormones Bicycling Blood Cell Count Blood Counts 030104 developmental biology Endocrinology Gene Expression Regulation Physical Fitness biology.protein Physical Endurance lcsh:Q business Homeostasis Genetics and Molecular Biology(all)
Zdroj:	PLoS ONE PLoS One, 13, 1-23 PLoS One, 13, 10, pp. 1-23 Van Staveren, S, Ten Haaf, T, Klöpping, M, Hilvering, B, Tinnevelt, G H, De Ruiter, K, Piacentini, M F, Roelands, B, Meeusen, R, De Koning, J J, Jansen, J J, Vrisekoop, N & Koenderman, L 2018, ' Multi-dimensional flow cytometry analysis reveals increasing changes in the systemic neutrophil compartment during seven consecutive days of endurance exercise ', PLoS ONE, vol. 13, no. 10, e0206175 . https://doi.org/10.1371/journal.pone.0206175 van Staveren, S, Ten Haaf, T, Klöpping, M, Hilvering, B, Tinnevelt, G H, de Ruiter, K, Piacentini, M F, Roelands, B, Meeusen, R, de Koning, J J, Jansen, J J, Vrisekoop, N & Koenderman, L 2018, ' Multi-dimensional flow cytometry analysis reveals increasing changes in the systemic neutrophil compartment during seven consecutive days of endurance exercise ', PLoS ONE, vol. 13, no. 10, e0206175, pp. 1-23 . https://doi.org/10.1371/journal.pone.0206175 PLoS ONE, Vol 13, Iss 10, p e0206175 (2018) PLoS ONE, 13(10):e0206175. Public Library of Science PLoS ONE, 13(10). Public Library of Science PLoS ONE, 13(10):e0206175, 1-23. Public Library of Science
ISSN:	1932-6203
DOI:	10.1371/journal.pone.0206175
Popis:	Endurance exercise is associated with a transient increase in neutrophil counts in the peripheral blood. Here we investigate the impact of intensified endurance exercise on the neutrophil compartment. We hypothesized that intensified endurance exercise leads to mobilization of neutrophil subsets, which are normally absent in the blood. Furthermore, we followed the potential build-up of neutrophil activation and the impact on overnight recovery of the neutrophil compartment during a seven-day cycling tour. The neutrophil compartment was studied in 28 healthy amateur cyclists participating in an eight-day strenuous cycling tour. Blood samples were taken at baseline, after 4 days and after 7 days of cycling. The neutrophil compartment was analyzed in terms of numbers and its phenotype by deep phenotyping of flow cytometry data with the multi-dimensional analysis method FLOOD. Repeated endurance exercise led to a gradual increase in total neutrophil counts over the days leading to a 1.26 fold-increase (95%CI 1.01-1.51 p = 0.0431) in the morning of day 8. Flow cytometric measurements revealed the appearance of 2 additional neutrophil subsets: CD16brightCD62Ldim and CD16dimCD62Lbright. A complex change in neutrophil phenotypes was present characterized by decreased expression of both CD11b and CD62L and marked increased expression of LAIR-1, VLA-4 and CBRM1/5. The changes in expression were found on all neutrophils present in the blood. Strikingly, in strong contrast to our findings during acute inflammation evoked by LPS challenge, these neutrophils did not upregulate classical degranulation markers. In fact, our FLOOD analysis revealed that the exercise induced neutrophil phenotype did not overlap with the neutrophil subsets arising upon acute inflammation. In conclusion, during multiple days of endurance exercise the neutrophil compartment does not regain homeostasis overnight. Thereby our study supports the concept of a build-up of inflammatory cues during repeated endurance exercise training, causing a prolonged change of the systemic neutrophil compartment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31f7c21a865c0818dc380b61180f2d5b https://pubmed.ncbi.nlm.nih.gov/30376575 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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