Reduced susceptibility to biocides in Acinetobacter baumannii: association with resistance to antimicrobials, epidemiological behaviour, biological cost and effect on the expression of genes encoding porins and efflux pumps
| Autor: | Jesús Rodríguez-Baño, Isabel Pujol Bajador, Álvaro Pascual Hernández, Simona Mihaela Iftimie, Antonio Gutiérrez-Pizarraya, Jordi Vila, Jerónimo Pachón, Luis Martínez Martínez, María de la Luz Cádiz-Gurrea, Teresa Nebreda-Mayoral, José Miguel Cisneros, Alfonso Del Arco Jiménez, Benito Regueiro |
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| Rok vydání: | 2015 |
| Předmět: |
Acinetobacter baumannii
Microbiology (medical) Genotype Tetracycline Gene Expression Porins Microbial Sensitivity Tests Drug resistance Microbiology Benzalkonium chloride Antibiotic resistance Drug Resistance Bacterial medicine Humans Pharmacology (medical) Pharmacology Doxycycline biology Membrane Transport Proteins biology.organism_classification Anti-Bacterial Agents Ciprofloxacin Infectious Diseases Efflux Energy Metabolism Acinetobacter Infections Disinfectants medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 70:3222-3229 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkv262 |
| Popis: | OBJECTIVES The objective of this study was to analyse whether there is an association between reduced susceptibility to biocides in Acinetobacter baumannii and (i) antimicrobial resistance (co-resistance), (ii) prevalent (epidemic) clones, (iii) changes in the fitness or (iv) expression of genes related to efflux pumps and porins. METHODS Susceptibility to biocides and antimicrobials was determined in 49 clonally unrelated isolates of A. baumannii. Biological cost, in terms of mean generation time, was determined by spectrophotometry. Quantitative real-time RT-PCR was used to determine the relative expression of genes encoding several efflux pumps and porins. RESULTS Reduced susceptibility to chlorhexidine digluconate, benzalkonium chloride and Irgasan(®) was associated with resistance to aminoglycosides, tetracycline and ciprofloxacin (P |
| Databáze: | OpenAIRE |
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