Comparative Proteome Analysis of Breast Cancer and Adjacent Normal Breast Tissues in Human
| Autor: | Hui-Jun Yang, Tian Yong Xing, Shi Shan Deng, Ruo Hong Xiong, You Guang Lu, Shang Qing Liu, Hong Ying Zhou, Bin Wen |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Models Molecular Proteomics Serum albumin Cathepsin D Breast Neoplasms Biochemistry Article Mass Spectrometry breast cancer Breast cancer Biomarkers Tumor Genetics medicine Humans Electrophoresis Gel Two-Dimensional Breast skin and connective tissue diseases Molecular Biology Polyacrylamide gel electrophoresis biology business.industry Tumor Protein Translationally-Controlled 1 Middle Aged Prognosis medicine.disease Gene Expression Regulation Neoplastic Computational Mathematics Matrix-assisted laser desorption/ionization 2D-PAGE Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Immunology Proteome biology.protein Cancer research biomarker Biomarker (medicine) Female molecular mechanism business |
| Zdroj: | Genomics, Proteomics & Bioinformatics |
| ISSN: | 1672-0229 |
| DOI: | 10.1016/s1672-0229(06)60029-6 |
| Popis: | Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS), incorporated with online database searching, were performed to investigate differential proteins of breast cancer and adjacent normal breast tissues. Considering that serum albumin is abundantly presented in normal control samples, 15 differential spots detected in 11 out of 12 (91.7%) breast cancer samples were identified by online SIENA-2DPAGE database searching and MALDI-TOF/TOF-MS analysis. The results indicate that pathological changes of breast cancer are concerned with augmentation of substance metabolism, promotion of proteolytic activity, decline of activity of some inhibitors of enzymes, and so on. Some important proteins involved in the pathological process of breast cancer with changed expression may be useful biomarkers, such as alpha-1-antitrypsin, EF-1-beta, cathepsin D, TCTP, SMT3A, RPS12, and PSMA1, among which SMT3A, RPS12, and PSMA1 were first reported for breast cancer in this study. |
| Databáze: | OpenAIRE |
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