Tumor cell metabolism correlates with resistance to gas plasma treatment: The evaluation of three dogmas
| Autor: | Anke Schmidt, Klaus-Dieter Weltmann, Julia Berner, Kristian Wende, Hans-Robert Metelmann, Grit Liebelt, Sanjeev Kumar Sagwal, Jonas Menz, Sander Bekeschus, Steffen Emmert, Thomas von Woedtke, Lars Boeckmann |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Aquaporin Biochemistry 03 medical and health sciences 0302 clinical medicine Physiology (medical) Cell Line Tumor medicine Cytotoxicity chemistry.chemical_classification Reactive oxygen species NADPH oxidase biology Chemistry Cancer NADPH Oxidases Hydrogen Peroxide medicine.disease 030104 developmental biology Catalase Cancer cell biology.protein Cancer research Plasma medicine Reactive Oxygen Species Oxidation-Reduction 030217 neurology & neurosurgery |
| Zdroj: | Free radical biologymedicine. 167 |
| ISSN: | 1873-4596 |
| Popis: | Gas plasma is a partially ionized gas increasingly recognized for targeting cancer. Several hypotheses attempt to explain the link between plasma treatment and cytotoxicity in cancer cells, all focusing on cellular membranes that are the first to be exposed to plasma-generated reactive oxygen species (ROS). One proposes high levels of aquaporins, membrane transporters of water and hydrogen peroxide, to mark tumor cell line sensitivity to plasma treatment. A second focuses on membrane-expression of redox-related enzymes such as NADPH oxidases (NOX) that may modify or amplify the effects of plasma-derived ROS, fueling plasma-induced cancer cell death. Another hypothesis is that the decreased cholesterol content of tumor cell membranes sensitizes these to plasma-mediated oxidation and subsequently, cytotoxicity. Screening 33 surface molecules in 36 tumor cell lines in correlation to their sensitivity to plasma treatment, the expression of aquaporins or NOX members could not explain the sensitivity but were rather associated with treatment resistance. Correlation with transporter or enzyme activity was not tested. Analysis of cholesterol content confirmed the proposed positive correlation with treatment resistance. Strikingly, the strongest correlation was found for baseline metabolic activity (Spearman r = 0.76). Altogether, these data suggest tumor cell metabolism as a novel testable hypothesis to explain cancer cell resistance to gas plasma treatment for further elucidating this innovative field's chances and limitations in oncology. |
| Databáze: | OpenAIRE |
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