Experimental axonopathy induced by immunization with Campylobacter jejuni lipopolysaccharide from a patient with Guillain-Barré syndrome
| Autor: | Beatrice Creati, Vincenzo Caporale, Paola Gandolfi, Antonio Di Muzio, Antonino Uncini, Vincenza Prencipe, Margherita Capasso, Mirella Luciani, Christina M. Caporale, Maria Vittoria De Angelis |
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| Rok vydání: | 2006 |
| Předmět: |
Lipopolysaccharides
Male Time Factors Lipopolysaccharide Blotting Western Immunology Polyradiculoneuropathy Gene Expression Enzyme-Linked Immunosorbent Assay G(M1) Ganglioside Guillain-Barre Syndrome Campylobacter jejuni Epitope Epitopes chemistry.chemical_compound Gangliosides medicine Animals Humans Immunology and Allergy Guillain-Barre syndrome biology medicine.disease biology.organism_classification Antibodies Bacterial Disease Models Animal Titer Neurology Immunization chemistry biology.protein Electrophoresis Polyacrylamide Gel lipids (amino acids peptides and proteins) Rabbits Neurology (clinical) Antibody Antiganglioside antibodies |
| Zdroj: | Journal of Neuroimmunology. 174:12-20 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/j.jneuroim.2005.12.005 |
| Popis: | New Zealand white rabbits were immunized with a lipopolysaccharide (LPS) extracted from a Campylobacter jejuni HS:19 strain isolated from a GBS patient expressing GM1 and GD1a-like epitopes, Freund's adjuvant (group I) and Freund's adjuvant plus keyhole lympet hemocyanin (KLH) (group II). Both groups showed high titers of anti-LPS and anti-GM1 and lower titers of anti-GD1b and anti-GD1a antibodies. Weakness and axonal degeneration in sciatic nerves was detected in 1/11 of group I and 6/7 of group II. This model replicates, at least in part, the pathogenetic process hypothesized in the human axonal GBS with antiganglioside antibodies post C. jejuni infection and indicates that KLH plays an additional role in neuropathy induction. |
| Databáze: | OpenAIRE |
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