Botulinum Toxin B Affects Neuropathic Pain but Not Functional Recovery after Peripheral Nerve Injury in a Mouse Model
| Autor: | Sara Marinelli, Alba Finocchiaro, Valentina Vacca, Flaminia Pavone, Siro Luvisetto, Federica De Angelis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine sciatic static index glia Health Toxicology and Mutagenesis Analgesic lcsh:Medicine Pharmacology weight bearing Toxicology walking track analysis Article Mice 03 medical and health sciences 0302 clinical medicine Peripheral Nerve Injuries medicine Animals Schwann cells Botulinum Toxins Type A nerve regeneration allodynia Analgesics business.industry lcsh:R Chronic pain spinal cord Nerve injury medicine.disease Sciatic Nerve Botulinum toxin Disease Models Animal 030104 developmental biology Allodynia Hyperalgesia immunohistochemistry Neuropathic pain Peripheral nerve injury Neuralgia Sciatic nerve medicine.symptom business Biomarkers 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Toxins; Volume 10; Issue 3; Pages: 128 Toxins Toxins, Vol 10, Iss 3, p 128 (2018) Toxins (Basel) 10 (2018). doi:10.3390/toxins10030128 info:cnr-pdr/source/autori:Finocchiaro, Alba; Marinelli, Sara; De Angelis, Federica; Vacca, Valentina; Luvisetto, Siro; Pavone, Flaminia/titolo:Botulinum Toxin B Affects Neuropathic Pain but Not Functional Recovery after Peripheral Nerve Injury in a Mouse Model/doi:10.3390%2Ftoxins10030128/rivista:Toxins (Basel)/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume:10 |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins10030128 |
| Popis: | Clinical use of neurotoxins from Clostridium botulinum is well established and is continuously expanding, including in treatment of pain conditions. Background: The serotype A (BoNT/A) has been widely investigated, and current data demonstrate that it induces analgesia and modulates nociceptive processing initiated by inflammation or nerve injury. Given that data concerning the serotype B (BoNT/B) are limited, the aim of the present study was to verify if also BoNT/B is able not only to counteract neuropathic pain, but also to interfere with inflammatory and regenerative processes associated with the nerve injury. Methods: As model of neuropathic pain, chronic constriction injury (CCI) of the sciatic nerve was performed in CD1 male mice. Mice were intraplantarly injected with saline (control) or BoNT/B (5 or 7.5 pg/mouse) into the injured hindpaw. For comparison, another mouse group was injected with BoNT/A (15 pg/mouse). Mechanical allodynia and functional recovery of the injured paw was followed for 101 days. Spinal cords and sciatic nerves were collected at day 7 for immunohistochemistry. Results and Conclusions: The results of this study show that BoNT/B is a powerful biological molecule that, similarly to BoNT/A, can reduce neuropathic pain over a long period of time. However, the analgesic effects are not associated with an improvement in functional recovery, clearly highlighting an important difference between the two serotypes for the treatment of this chronic pain state. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|