Digitonin-sensitive LHCII enlarges the antenna of Photosystem I in stroma lamellae of Arabidopsis thaliana after far-red and blue-light treatment
| Autor: | Rob B. M. Koehorst, Emilie Wientjes, Anniek Oosterwijk, Peter Bos, Herbert van Amerongen, Arjen N. Bader, John Philippi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine ITIS Middelware Photosystem II Light Arabidopsis Light-Harvesting Protein Complexes Biophysics Digitonin Photosynthesis Photosystem I 01 natural sciences Biochemistry Fluorescence spectroscopy Light-harvesting complex 03 medical and health sciences Arabidopsis thaliana Phosphorylation Photosystem VLAG biology Photosystem I Protein Complex Chemistry IT Design Excitation-energy transfer Photosystem II Protein Complex Far-red Time-resolved fluorescence Cell Biology biology.organism_classification State transition 030104 developmental biology Spectrometry Fluorescence Biofysica Energy Transfer EPS 010606 plant biology & botany |
| Zdroj: | Biochimica et Biophysica Acta-Bioenergetics 1860 (2019) 8 Biochimica et Biophysica Acta-Bioenergetics, 1860(8), 651-658 |
| ISSN: | 0005-2728 |
| DOI: | 10.1016/j.bbabio.2019.07.001 |
| Popis: | Light drives photosynthesis. In plants it is absorbed by light-harvesting antenna complexes associated with Photosystem I (PSI) and photosystem II (PSII). As PSI and PSII work in series, it is important that the excitation pressure on the two photosystems is balanced. When plants are exposed to illumination that overexcites PSII, a special pool of the major light-harvesting complex LHCII is phosphorylated and moves from PSII to PSI (state 2). If instead PSI is over-excited the LHCII complex is dephosphorylated and moves back to PSII (state 1). Recent findings have suggested that LHCII might also transfer energy to PSI in state 1. In this work we used a combination of biochemistry and (time-resolved) fluorescence spectroscopy to investigate the PSI antenna size in state 1 and state 2 for Arabidopsis thaliana. Our data shows that 0.7 ± 0.1 unphosphorylated LHCII trimers per PSI are present in the stroma lamellae of state-1 plants. Upon transition to state 2 the antenna size of PSI in the stroma membrane increases with phosphorylated LHCIIs to a total of 1.2 ± 0.1 LHCII trimers per PSI. Both phosphorylated and unphosphorylated LHCII function as highly efficient PSI antenna. |
| Databáze: | OpenAIRE |
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