Borrelia burgdorferi Induces TLR2-Mediated Migration of Activated Dendritic Cells in an Ex Vivo Human Skin Model

Autor: Teunis B.H. Geijtenbeek, Tom van der Poll, Alex Wagemakers, Cornelis van 't Veer, Wouter J. van der Pot, Anneke Oei, Joppe W. Hovius, Lauren M.K. Mason, Kalam Ahmed
Přispěvatelé: Other departments, Center of Experimental and Molecular Medicine, Infectious diseases, Experimental Immunology
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Physiology
Biopsy
lcsh:Medicine
Human skin
Pathology and Laboratory Medicine
0302 clinical medicine
Spectrum Analysis Techniques
Cell Movement
Immune Physiology
Medicine and Health Sciences
lcsh:Science
Routes of Administration
Skin
Lyme Disease
Innate Immune System
Multidisciplinary
biology
Spirochetes
integumentary system
Pattern recognition receptor
Cell migration
hemic and immune systems
Flow Cytometry
Bacterial Pathogens
Cell Motility
Medical Microbiology
Spectrophotometry
Cytokines
Cytophotometry
Pathogens
Anatomy
Integumentary System
Research Article
Borrelia Burgdorferi
Cell Survival
Immunology
Surgical and Invasive Medical Procedures
chemical and pharmacologic phenomena
Cell Migration
Research and Analysis Methods
Microbiology
03 medical and health sciences
Immune system
Intradermal Injections
Humans
Borrelia burgdorferi
Microbial Pathogens
Pharmacology
Bacteria
Borrelia
lcsh:R
Organisms
Biology and Life Sciences
Dendritic cell
Dendritic Cells
Cell Biology
Molecular Development
biology.organism_classification
Toll-Like Receptor 2
TLR2
030104 developmental biology
Immune System
lcsh:Q
Ex vivo
Biomarkers
030215 immunology
Developmental Biology
Zdroj: PLoS ONE, 11(10). Public Library of Science
PLoS ONE
PLoS ONE, Vol 11, Iss 10, p e0164040 (2016)
Popis: Borrelia burgdorferi is transmitted into the skin of the host where it encounters and interacts with two dendritic cell (DC) subsets; Langerhans cells (LCs) and dermal DCs (DDCs). These cells recognize pathogens via pattern recognition receptors, mature and migrate out of the skin into draining lymph nodes, where they orchestrate adaptive immune responses. In order to investigate the response of skin DCs during the early immunopathogenesis of Lyme borreliosis, we injected B. burgdorferi intradermally into full-thickness human skin and studied the migration of DCs out of the skin, the activation profile and phenotype of migrated cells. We found a significant increase in the migration of LCs and DDCs in response to B. burgdorferi. Notably, migration was prevented by blocking TLR2. DCs migrated from skin inoculated with higher numbers of spirochetes expressed significantly higher levels of CD83 and produced pro-inflammatory cytokines. No difference was observed in the expression of HLA-DR, CD86, CD38, or CCR7. To conclude, we have established an ex vivo human skin model to study DC-B. burgdorferi interactions. Using this model, we have demonstrated that B. burgdorferi-induced DC migration is mediated by TLR2. Our findings underscore the utility of this model as a valuable tool to study immunity to spirochetal infections.
Databáze: OpenAIRE
Externí odkaz: