Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis
| Autor: | Inés Stella, Marcela Barrios, Analía Meilerman Abuelafia, Alfredo Daniel Vitullo, Silvia Galliano, Nicolas A. Fraunhoffer |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Pathology medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Adolescent Inmunología Endometriosis Ovary Biology Stem cell marker Brief Communication Andrology DEAD-box RNA Helicases Young Adult DDX4 Obstetrics and Gynaecology medicine Biomarkers Tumor Humans Proliferation Marker OVARIAN STEM CELLS Ovarian Neoplasms Stem Cells OVARIAN ENDOMETRIOSIS Obstetrics and Gynecology Membrane Proteins RNA-Binding Proteins purl.org/becyt/ford/3.1 [https] Middle Aged medicine.disease Ovarian endometriosis Ovarian stem cells Medicina Básica medicine.anatomical_structure Germ Cells IFITM3 Oncology Ovarian Endometriosis Female purl.org/becyt/ford/3 [https] Ovarian Endometriotic Cyst Stem cell Germ cell |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Journal of Ovarian Research |
| DOI: | 10.1186/s13048-015-0193-8 |
| Popis: | Background: Endometriosis is a gynaecological disorder that affects 6-10 % of female population. It is characterized by the presence of endometrial tissue outside the uterus, most often in the pelvic peritoneum or ovaries. Recent studies have indicated that mesenchymal endometrial stem cells might get involved in endometriosis progression. Although germ line stem cells have been proved to exist in the ovary, their involvement in ovarian endometriosis has not been investigated. In this preliminary report we aimed to identify germinal stem cell markers in ovarian endometriosis. Findings: Ten paraffin-embedded ovarian endometriosis samples were screened for germ cell-specific proteins DDX4 (VASA) and IFITM3, and its relation with stem cell marker OCT4, proliferation marker PCNA and estrogen receptor alpha (ESR1), by immunohistochemistry, immunofluorescence and PCR. DDX4 and IFITM3 proteins were expressed in isolated cells and clusters of cells in the cortical region of ovarian endometriotic cysts. DDX4 and IFITM3 co-localized in cells from endometriotic stroma, and DDX4/IFITM3-expressing cells were positive for ESR1, OCT4 and PCNA. No cells expressing neither DDX4 nor IFITM3 were detected in normal endometrial tissue. Conclusion: The identification of germ cell-specific proteins DDX4 and IFITM3 provides the first evidence of ovarian-sourced cells in ovarian endometriotic lesions and opens up new directions towards understanding the still confusing pathogenesis of endometriosis. Fil: Fraunhoffer Navarro, Nicolas Alejandro. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Meilerman Abuelafia, Miriam Analia. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina Fil: Stella, Inés. Universidad Maimónides; Argentina Fil: Galliano, Silvia. Universidad Maimónides; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; Argentina Fil: Barrios, Marcela. Universidad Maimónides; Argentina Fil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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