New antibodies immobilization system into a graphite–polysulfone membrane for amperometric immunosensors
| Autor: | Esteve Fàbregas, Samuel Sánchez Ordóñez |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Analyte
Polymers Biomedical Engineering Biophysics Antigen-Antibody Complex Biosensing Techniques Antibodies Coated Materials Biocompatible Electrochemistry Animals Sulfones Immunoassay Detection limit Chromatography Chemistry Substrate (chemistry) Equipment Design General Medicine Amperometry Equipment Failure Analysis Membrane Linear range Immunoglobulin G Electrode Graphite Adsorption Rabbits Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 22:965-972 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2006.03.022 |
| Popis: | Polysulfone membrane is used for the first time for the preparation of electrochemical immunosensors. A disposable immunosensor based on a porous conductor polymer graphite-polysulfone-electrode has been developed using a phase inversion technique for the determination of anti-rabbit IgG (anti-RIgG) as a model analyte. To construct the sensor, a conductor membrane was deposited on the surface of working graphite-epoxy composite (GEC) electrode. The membrane was characterized by SEM. This sensor was based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). Incubation parameters were optimized in this work. The immunological reaction was detected using an enzymatic-labeling procedure (HRP enzyme) combined with the amperometric detection using H(2)O(2) as substrate and hydroquinone as mediator. This sensor shows stability during a week and a good reproducibility. The current was monitored amperometrically at -0.1 V versus SCE and this method showed a linear range of the anti-RIgG from 1 to 6 microg/ml. The detection limit was determined to be 0.77 microg/ml. |
| Databáze: | OpenAIRE |
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