Characterisation of lymphocyte subpopulations in infantile haemangioma
| Autor: | Helen D. Brasch, Tinte Itinteang, Elysia M S Tan, Daria A. Chudakova, Reginald W. Marsh, Paul F. Davis, Jonathan C. Dunne, Swee T. Tan |
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| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty CD3 Complex Lymphocyte CD3 Population B-Lymphocyte Subsets CD34 Real-Time Polymerase Chain Reaction Immunophenotyping Pathology and Forensic Medicine T-Lymphocyte Subsets Tandem Mass Spectrometry Biomarkers Tumor medicine Humans Cell Lineage RNA Messenger education In Situ Hybridization Cell Proliferation education.field_of_study biology Reverse Transcriptase Polymerase Chain Reaction Endothelial Cells Infant General Medicine T lymphocyte Antigens CD20 Immunohistochemistry Staining Thymocyte Phenotype medicine.anatomical_structure biology.protein Leukocyte Common Antigens Thy-1 Antigens Hemangioma |
| Zdroj: | Journal of Clinical Pathology. 68:812-818 |
| ISSN: | 1472-4146 0021-9746 |
| Popis: | AimsInterstitial CD45+ cells and T lymphocytes have previously been demonstrated within infantile haemangioma (IH). This study investigated the expression of B and T lymphocyte markers by the CD45+ population, and the expression of Thy-1, a marker of thymocyte progenitors, which have the ability to give rise to both B and T cells.MethodsImmunohistochemical (IHC) staining was performed on proliferating and involuted IHs for the expression of CD45, CD3, CD20, CD79a, Thy-1 and CD34. The presence of mRNA corresponding toCD45, CD3G, CD20andThy-1was confirmed by reverse transcriptase-polymerase chain reaction in snap-frozen IH tissues. Cell counting of 3,3-diaminobenzidine IHC-stained slides was performed on CD45+ only cells and dually stained CD45+/CD3+ cells or CD45+/CD20+ cells and analysed statistically. In situ hybridisation and mass spectrometry were also performed to confirm the presence and abundance of Thy-1, respectively.ResultsIHC staining showed a subpopulation of CD45+ interstitial cells that expressed the T lymphocyte marker, CD3, and another subpopulation that expressed the B lymphocyte marker, CD20, in proliferating and diminished in involuted IHs. The abundant expression of Thy-1 on the endothelium of proliferating, but not involuted IH, was demonstrated by IHC staining and confirmed by in situ hybridisation and mass spectrometry.ConclusionsBoth B and T lymphocytes are present within the interstitium of proliferating and involuted IH. The expression of Thy-1 by the endothelium suggests that B and T cells in IH may have originated from within the lesion, rather than migrating from the peripheral circulation. |
| Databáze: | OpenAIRE |
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