Process-independent quantitative assessment of residual biological contamination of medical devices reprocessed in washer-disinfectors
| Autor: | Dana Gášková, K. Chládková, V. Vacata, Jürgen Gebel, Karel Sigler |
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| Rok vydání: | 2005 |
| Předmět: |
Washer
Cross Infection Soil test Analytical chemistry Soil science General Medicine Contamination Residual complex mixtures Microbiology Stripping (fiber) Disinfection Soil Scientific method Equipment Reuse Environmental science media_common.cataloged_instance Equipment Contamination Humans European Union European union Soil microbiology Soil Microbiology media_common |
| Zdroj: | Folia microbiologica. 50(3) |
| ISSN: | 0015-5632 |
| Popis: | A method based on measuring a soil-induced fluorescence intensity response of 1,8-anilinonaphthalene sulfonate at two fixed wavelengths (460 and 510 nm) was used for determining residual contamination on test soil carriers simulating medical devices after passage through a hospital washer-disinfector. The fluorescence response can be satisfactorily calibrated to soil levels as low as approximately 1 microg/L. Practical tests were performed in two hospitals with washer-disinfectors of 3 types and with several chemical or enzymic cleansers-disinfectants. In combination with the previously developed system of standardized test soil carriers simulating both easily and poorly accessible parts of soiled medical devices, the liver-lactose-oil test soil and an efficient sonication procedure for stripping the residual soil off the carriers, this soil detection method permits the detection of very low contamination levels. |
| Databáze: | OpenAIRE |
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