PLGA/alginate composite microspheres for hydrophilic protein delivery
| Autor: | Peng Zhai, Xiongbiao Chen, David J. Schreyer |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Materials science
Biocompatibility Alginates Scanning electron microscope Composite number chemistry.chemical_element Bioengineering macromolecular substances Calcium Antibodies Cell Line Microsphere Biomaterials Mice chemistry.chemical_compound Drug Delivery Systems Glucuronic Acid Polylactic Acid-Polyglycolic Acid Copolymer Pulmonary surfactant Materials Testing Animals Lactic Acid Bovine serum albumin biology Hexuronic Acids technology industry and agriculture Serum Albumin Bovine Microspheres PLGA chemistry Biochemistry Chemical engineering Mechanics of Materials biology.protein Cattle Laminin Rabbits Hydrophobic and Hydrophilic Interactions Polyglycolic Acid |
| Zdroj: | Materials Science and Engineering: C. 56:251-259 |
| ISSN: | 0928-4931 |
| Popis: | Poly(lactic-co-glycolic acid) (PLGA) microspheres and PLGA/alginate composite microspheres were prepared by a novel double emulsion and solvent evaporation technique and loaded with bovine serum albumin (BSA) or rabbit anti-laminin antibody protein. The addition of alginate and the use of a surfactant during microsphere preparation increased the encapsulation efficiency and reduced the initial burst release of hydrophilic BSA. Confocal laser scanning microcopy (CLSM) of BSA-loaded PLGA/alginate composite microspheres showed that PLGA, alginate, and BSA were distributed throughout the depths of microspheres; no core/shell structure was observed. Scanning electron microscopy revealed that PLGA microspheres erode and degrade more quickly than PLGA/alginate composite microspheres. When loaded with anti-laminin antibody, the function of released antibody was well preserved in both PLGA and PLGA/alginate composite microspheres. The biocompatibility of PLGA and PLGA/alginate microspheres were examined using four types of cultured cell lines, representing different tissue types. Cell survival was variably affected by the inclusion of alginate in composite microspheres, possibly due to the sensitivity of different cell types to excess calcium that may be released from the calcium cross-linked alginate. |
| Databáze: | OpenAIRE |
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