Diffuse reflectance spectroscopy accurately discriminates early and advanced grades of fatty liver in mice
| Autor: | Galileo Escobedo, Diego A. Fabila-Bustos, Adriana Campos-Espinosa, José Manuel de la Rosa Vázquez, Alma Rosa Valor Reed, Suren Stolik Isakina, Eduardo J. Arista Romeu, Ivette Irais Romero-Bello, Javier Moreno-González, Carolina Guzmán |
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| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty Cirrhosis Biomedical Engineering Chronic liver disease Gastroenterology Biomaterials Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Non-alcoholic Fatty Liver Disease Internal medicine Biopsy Nonalcoholic fatty liver disease medicine Animals Oil Red O medicine.diagnostic_test business.industry Spectrum Analysis Fatty liver Equipment Design medicine.disease Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Mice Inbred C57BL Disease Models Animal Liver chemistry 030220 oncology & carcinogenesis Liver biopsy 030211 gastroenterology & hepatology Steatosis business |
| Zdroj: | Journal of Biomedical Optics. 23:1 |
| ISSN: | 1083-3668 |
| Popis: | Nonalcoholic fatty liver disease (NAFLD) ranges from steatosis to nonalcoholic steatohepatitis and cirrhosis. Liver biopsy, considered the gold standard to diagnose NAFLD, shows significantly high rates of interobserver variability. Thus there is a need to develop tools that accurately categorize mild and advanced grades of steatosis in order to identify patients at higher risk of developing chronic liver disease. Diffuse reflectance spectroscopy (DRS) has proved to be useful in grading liver fibrosis and cirrhosis, without having been implemented for steatosis. We aim to categorize early and advanced stages of liver steatosis in a methionine-choline deficient (MCD) mouse model. C57bl/6 mice are fed either methionine-choline control or MCD diet during 2 or 8 weeks to induce mild and advanced steatosis. Liver samples are obtained and steatosis is evaluated by oil red O staining. Diffuse reflectance spectra are directly measured on ex vivo liver specimens, in a wavelength range of 400 to 800 nm. DRS is able to discriminate between early or advanced steatosis and healthy hepatic tissue with negligible error while showing high average sensitivity and specificity (0.94 and 0.95, respectively). Our results suggest that liver steatosis can be accurately evaluated by DRS, highlighting the importance of applied spectroscopic methods in assessing NAFLD. |
| Databáze: | OpenAIRE |
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