In Vitro Exposure to Highly Cytopathic HIV-1 X4 Strains Increases Expression of Mucosa-Associated Integrins on CD4+ T Cells
| Autor: | James E. Cummins, Jiri Mestecky, William J. Bunn, Stacy Hall, Hollie Hale Donze, Susan Jackson |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
CD4-Positive T-Lymphocytes
Integrins αEβ7 integrin Receptor expression Integrin HIV Infections CXCR4 Cytopathogenic Effect Viral Virology T lymphocyte Cytotoxic T cell Humans L-Selectin Lymphocyte homing receptor mucosa Cells Cultured Mucous Membrane biology virus diseases Molecular biology CD4 α4β7 biology.protein HIV-1 L-selectin cytopathic Homing (hematopoietic) |
| Zdroj: | Virology. 280(2):262-272 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.2000.0781 |
| Popis: | To determine whether infection with HIV-1 strains of different tropisms would influence expression of the mucosa-associated integrins alpha 4 beta 7 and alpha E beta 7 or the lymph node homing receptor L-selectin on peripheral T lymphocytes, cells were infected with the CXCR4-tropic (X4)/syncytium-inducing (SI) HIV-1(IIIB) strain or with X4/SI or CCR5-tropic (R5)/non-SI (NSI) primary human isolates. Flow cytometric analyses of CD4(+) T cells from cultures infected with HIV-1(IIIB) and one X4/SI primary HIV-1 isolate revealed a significant increase in surface expression of alpha 4 beta 7 and alpha E beta 7 12 days after infection. L-selectin expression was not significantly affected on CD4(+) T cells. However, infection with another X4/SI and two R5/NSI primary HIV-1 isolates did not significantly alter homing receptor expression on CD4(+) T cells. Since a higher degree of CD4 cytopathicity occurred in those cultures having increased integrin expression, these data suggest that significantly altered mucosal homing receptor expression on CD4(+) T cells may result as a "bystander" effect after infection with some cytopathic isolates of HIV-1. |
| Databáze: | OpenAIRE |
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