Quantitative Liquid Chromatography-Nanoelectrospray Ionization-High Resolution Tandem Mass Spectrometry Analysis of Acrolein-DNA Adducts and Etheno-DNA Adducts in Oral Cells from Cigarette Smokers and Non-smokers
| Autor: | Valeria Guidolin, Jing Yang, Viviana Paiano, Silvia Balbo, Stephen S. Hecht, Laura A. Maertens |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
010501 environmental sciences Toxicology Tandem mass spectrometry 01 natural sciences Article Adduct Cell Line Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound DNA Adducts Tandem Mass Spectrometry medicine Humans Nucleotide Oral mucosa Acrolein Carcinogen 030304 developmental biology 0105 earth and related environmental sciences chemistry.chemical_classification 0303 health sciences Chromatography Smokers Molecular Structure General Medicine Non-Smokers medicine.anatomical_structure chemistry DNA Chromatography Liquid |
| Zdroj: | Chem Res Toxicol |
| Popis: | Cigarette smoking is an important source of human exposure to toxicants and carcinogens and contributes significantly to cancer morbidity and mortality worldwide. Acrolein, a widespread environmental pollutant, is present in relatively high amounts in cigarette smoke and can react directly with DNA to form DNA adducts, which serve as important biomarkers for the assessment of exposure to acrolein and its potential role in smoking related cancer. Etheno-DNA adducts are promutagenic DNA lesions that can derive from exogenous chemicals as well as endogenous sources, including lipid peroxidation. In this study, we developed a combined method for the quantitation of (6R/S)-3-(2′-deoxyribos-1′-yl)-5,6,7,8,-tetrahydro-6-hydroxypyrimido[1,2-a]purine-10(3H)-one (α-OH-Acr-dGuo), (8R/S)-3-(2′-deoxyribos-1′-yl)-5,6,7,8,-tetrahydro-8-hydroxypyrimido[1,2-a]purine-10(3H)-one (γ-OH-Acr-dGuo), 1,N(6)-etheno-dAdo (εdAdo) and 3,N(4)-etheno-dCyd (εdCyd) adducts in oral rinse and cytobrush DNA from smokers and non-smokers by liquid chromatography-nanoelelctrospray ionization-high resolution tandem mass spectrometry (LC-NSI-HRMS/MS). For oral rinse samples, there was a statistically significant difference between the levels of α-OH-Acr-dGuo, γ-OH-Acr-dGuo, εdAdo and εdCyd in smokers (12.1 ± 17.9, 163 ± 227, 182 ± 568, and 194 ± 400 adducts/10(9) nucleotides, respectively) and non-smokers (1.85 ± 2.08, 5.95 ± 4.23, 7.69 ± 11.7, and 6.07 ± 10.9 adducts/10(9) nucleotides, respectively). For cytobrush samples, there was a statistically significant difference between the levels of γ-OH-Acr-dGuo and εdAdo in smokers (259 ± 540, and 82.9 ± 271 adducts/10(9) nucleotides, respectively) and non-smokers (7.37 ± 5.09, and 16.2 ± 30.2 adducts/10(9) nucleotides, respectively), but not for α-OH-Acr-dGuo and εdCyd. Our results demonstrate that oral mucosa cells are an excellent source of material for evaluating DNA adducts to be used as biomarkers of tobacco smoke exposure and molecular changes potentially related to cancer. |
| Databáze: | OpenAIRE |
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