Zinc Supplementation with or without Additional Micronutrients Does Not Affect Peripheral Blood Gene Expression or Serum Cytokine Level in Bangladeshi Children
| Autor: | Sant-Rayn Pasricha, Cavan Bennett, Rosie Watson, Rahvia Alam Sthity, Afsana Mim Khandaker, Christine McDonald, Nancy F. Krebs, Ricardo Ataide, Thomas Hayman, Daniela Amann-Zalcenstein, Nawaf Yassi, Kazi Munisul Islam, Katharina Stracke, Peter Hickey, Jamie Westcott, Janet C. King, Julie Long, Robert E. Black, Md. Munirul Islam |
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| Rok vydání: | 2021 |
| Předmět: |
Male
and promotion of well-being medicine.medical_treatment Physiology Gene Expression immunology transcriptomics TX341-641 Micronutrients Wasting Pediatric Bangladesh Nutrition and Dietetics zinc RNA sequencing Micronutrient Cytokine Infectious Diseases Cytokines Female Zero Hunger medicine.symptom Powders Tablets Clinical Trials and Supportive Activities chemistry.chemical_element Zinc Placebo Article Immune system Food Sciences Clinical Research Complementary and Integrative Health medicine Genetics Humans 3.3 Nutrition and chemoprevention Nutrition Nutrition. Foods and food supply business.industry Prevention Infant medicine.disease Prevention of disease and conditions Malnutrition Good Health and Well Being chemistry Dietary Supplements Zinc deficiency business Food Science |
| Zdroj: | Nutrients, vol 13, iss 10 Nutrients, Vol 13, Iss 3516, p 3516 (2021) Nutrients Volume 13 Issue 10 |
| Popis: | Preventive zinc supplementation provided as a stand-alone dispersible tablet, or via home fortification as multiple micronutrient powders (MNPs), has been considered a potential strategy to prevent zinc deficiency and improve health (including immune) outcomes among children in low- and middle-income countries. However, the impact of zinc supplementation on immune profiles has not been well characterized. We sought to define the effect of zinc supplementation on peripheral blood gene expression and cytokine levels among young children in Dhaka, Bangladesh. In a sub-study of a large randomized, controlled, community-based efficacy trial where children 9–11 months of age received one of the following interventions on a daily basis for 24 weeks: (1) MNPs containing 10 mg of zinc (2) dispersible tablet containing 10 mg zinc or (3) placebo powder, we used RNA sequencing to profile the peripheral blood gene expression, as well as highly sensitive multiplex assays to detect cytokine profiles. We profiled samples from 100 children enrolled in the parent trial (zinc MNPs 28, zinc tablets 39, placebo 33). We did not detect an effect from either zinc intervention on differential peripheral blood gene expression at the end of the intervention, or an effect from the intervention on changes in gene expression from baseline. We also did not detect an effect from either intervention on cytokine concentrations. Exploratory analysis did not identify an association between undernutrition (defined as stunting, underweight or wasting) and peripheral blood gene expression. Zinc interventions in children did not produce a gene expression or cytokine signature in the peripheral blood. However, this study demonstrates a proof of principle that sensitive multi-omic techniques can be applied to samples collected in field studies. |
| Databáze: | OpenAIRE |
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