Redox-dependent control of i-Motif DNA structure using copper cations
| Autor: | Andrew J. Gates, Mahmoud A. S. Abdelhamid, Zoë A. E. Waller, Myles R. Cheesman, László Fábián, Colin J. Macdonald |
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| Rok vydání: | 2018 |
| Předmět: |
Models
Molecular Base pair chemistry.chemical_element Biology 010402 general chemistry 01 natural sciences Oxygen Redox Cytosine chemistry.chemical_compound Chemical Biology and Nucleic Acid Chemistry Cations Genetics Chelation Nucleotide Motifs Base Pairing 010405 organic chemistry Ligand DNA Copper 0104 chemical sciences Crystallography chemistry Oxidation-Reduction |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gky390 |
| Popis: | Previous computational studies have shown that Cu+ can act as a substitute for H+ to support formation of cytosine (C) dimers with similar conformation to the hemi-protonated base pair found in i-motif DNA. Through a range of biophysical methods, we provide experimental evidence to support the hypothesis that Cu+ can mediate C–C base pairing in i-motif DNA and preserve i-motif structure. These effects can be reversed using a metal chelator, or exposure to ambient oxygen in the air that drives oxidation of Cu+ to Cu2+, a comparatively weak ligand. Herein, we present a dynamic and redox-sensitive system for conformational control of an i-motif forming DNA sequence in response to copper cations. |
| Databáze: | OpenAIRE |
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