Blastocyst transfer following intracytoplasmic injection of ejaculated, epididymal or testicular spermatozoa
| Autor: | Aycan Isiklar, Ramazan Mercan, Alp Nuhoglu, Basak Balaban, Senai Aksoy, Cengiz Alatas, Bulent Urman |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male medicine.medical_treatment Biology Intracytoplasmic sperm injection Andrology Embryonic and Fetal Development Pregnancy Testis medicine Humans Ejaculation Sperm Injections Intracytoplasmic Blastocyst reproductive and urinary physiology Epididymis Azoospermia urogenital system Rehabilitation Blastocyst Transfer Pregnancy Outcome Obstetrics and Gynecology Embryo culture Embryo Oligospermia Embryo Transfer medicine.disease Spermatozoa Embryo transfer medicine.anatomical_structure Reproductive Medicine embryonic structures Female |
| Zdroj: | Human Reproduction. 16:125-129 |
| ISSN: | 1460-2350 0268-1161 |
| Popis: | Recent studies indicate a strong paternal influence on embryo development and progression of the embryo to the blastocyst stage. The aim of this study was to compare, during extended culture, the in-vitro development of embryos resulting from intracytoplasmic sperm injection (ICSI) of ejaculated spermatozoa (group 1, n = 347), epididymal (group 2, n = 22) or testicular (group 3, n = 18) spermatozoa from obstructive azoospermic and testicular spermatozoa from non-obstructive azoospermic (group 4, n = 31) subjects. Fertilization and blastocyst formation rates were significantly lower in group 4 (P < 0.05). The incidence of expanded and hatching blastocysts was significantly lower in group 4 (P < 0.05). Overall in 93.2% ejaculate ICSI cycles, blastocysts were transferred on day 5. This was significantly higher than the 62% day 5 transfers in the non-obstructive azoospermic group (P < 0.05). Implantation rate per embryo was significantly higher in the ejaculate ICSI group compared with the other groups (P < 0.05). Clinical pregnancy per transfer was similar between groups; however, significantly fewer multiple pregnancies were encountered in the non-obstructive azoospermic group (P < 0.01). In conclusion, the source of the spermatozoa, most likely to be indicative of the severity of spermatogenic disorder, affects the rate of blastocyst formation and blastocyst implantation. Spermatozoa from non-obstructive azoospermic subjects, when utilized for ICSI, result in embryos that progress to the blastocyst stage at a lower and slower rate and implant less efficiently. |
| Databáze: | OpenAIRE |
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