Human Dental Pulp Stem Cells Improve Left Ventricular Function, Induce Angiogenesis, and Reduce Infarct Size in Rats with Acute Myocardial Infarction

Autor: Rafael Payá, Pilar Sepúlveda, Mauro Llop, Vicente Mirabet, Amparo Ruiz, Ana Armiñán, Elisa Lledó, Francisco Carbonell-Uberos, José Manuel García-Verdugo, Jorge Sanchez-Torrijos, M Dolores Miñana, José Anastasio Montero, Carolina Gandía
Rok vydání: 2007
Předmět:
Adult
Male
Pathology
medicine.medical_specialty
Adolescent
Angiogenesis
medicine.medical_treatment
Myocytes
Smooth Muscle
Cell- and Tissue-Based Therapy
Myocardial Infarction
Neovascularization
Physiologic
Biology
stem cell therapy
ventricular remodeling
Ventricular Function
Left
Rats
Nude
left ventricular function
Dental pulp stem cells
medicine
Animals
Humans
Myocytes
Cardiac
Myocardial infarction
Ventricular remodeling
Dental Pulp
Cell Proliferation
Ultrasonography
mesenchymal stem cells
Stem Cells
Cardiac muscle
Cell Differentiation
Mesenchymal Stem Cells
Amniotic stem cells
Cell Biology
Stem-cell therapy
Anatomy
medicine.disease
dental pulp stem cells
Rats
Retroviridae
medicine.anatomical_structure
Molecular Medicine
Stem cell
Retroviridae Infections
Stem Cell Transplantation
Developmental Biology
Zdroj: Stem Cells
r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe
instname
STEM CELLS
r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
Centro de Investigación Principe Felipe (CIPF)
ISSN: 1549-4918
1066-5099
DOI: 10.1634/stemcells.2007-0484
Popis: Human dental pulp contains precursor cells termed dental pulp stem cells (DPSC) that show self-renewal and multilineage differentiation and also secrete multiple proangiogenic and antiapoptotic factors. To examine whether these cells could have therapeutic potential in the repair of myocardial infarction (MI), DPSC were infected with a retrovirus encoding the green fluorescent protein (GFP) and expanded ex vivo. Seven days after induction of myocardial infarction by coronary artery ligation, 1.5 × 106 GFP-DPSC were injected intramyocardially in nude rats. At 4 weeks, cell-treated animals showed an improvement in cardiac function, observed by percentage changes in anterior wall thickening left ventricular fractional area change, in parallel with a reduction in infarct size. No histologic evidence was seen of GFP+ endothelial cells, smooth muscle cells, or cardiac muscle cells within the infarct. However, angiogenesis was increased relative to control-treated animals. Taken together, these data suggest that DPSC could provide a novel alternative cell population for cardiac repair, at least in the setting of acute MI. Disclosure of potential conflicts of interest is found at the end of this article.
Databáze: OpenAIRE
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