Immunohistochemical Localization of Chondroitin Sulfate, Chondroitin Sulfate Proteoglycan, Heparan Sulfate Proteoglycan, Entactin, and Laminin in Basement Membranes of Postnatal Developing and Adult Rat Lungs
| Autor: | John R. Couchman, Kimberly K. Burch, Kevin J. McCarthy, Philip L. Sannes, Jody Khosla |
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| Rok vydání: | 1993 |
| Předmět: |
Pulmonary and Respiratory Medicine
Aging animal structures Clinical Biochemistry Perlecan Basement Membrane Immunoenzyme Techniques Extracellular matrix chemistry.chemical_compound Laminin medicine Animals Chondroitin sulfate Lung Molecular Biology Basement membrane Membrane Glycoproteins biology Chondroitin Sulfates Cell Biology Immunohistochemistry Molecular biology Rats carbohydrates (lipids) medicine.anatomical_structure Animals Newborn Chondroitin Sulfate Proteoglycans chemistry Proteoglycan Biochemistry Chondroitin sulfate proteoglycan biology.protein Proteoglycans Heparitin Sulfate Heparan Sulfate Proteoglycans Immunostaining |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 8:245-251 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/ajrcmb/8.3.245 |
| Popis: | Histologic preparations of lungs from 1-, 5-, 10-, 18-, and 25-day-old postnatal and adult rats were examined immunohistochemically with antibodies specific against chondroitin sulfate (CS), basement membrane chondroitin sulfate proteoglycan (BM-CSPG), heparan sulfate proteoglycan (HSPG), entactin, and laminin. A monoclonal antibody specific for the glycosaminoglycan portion (CS) of CSPG and a monoclonal antibody against the core protein of CSPG were used in an immunoperoxidase sequence to stain extracellular matrix (ECM) components of pulmonary basement membranes (BMs). Anti-CS stained airway BM strongly and alveolar BM weakly in the adult rat lung, as well as in vascular and airway adventitia. In developing lungs, immunoreactivity was strong in all ECM sites, including BM, at day 1 postnatal, and progressively diminished thereafter except in vascular and airway adventitia. Anti-CSPG stained alveolar, airway, and vascular BMs, in addition to smooth muscle external laminae (EL), in the adult and developing rat. Immunostaining for CSPG required hyaluronidase digestion, whereas CS staining was lost with the same treatment. A polyclonal antibody to the core protein of HSPG was found to be similarly distributed to CSPG by immunoperoxidase staining in adult and developing rat lungs, with the notable exception that little immunoreactivity for HSPG was found in smooth muscle EL. Commercially obtained polyclonal antibodies to entactin and laminin gave immunostaining comparable to that seen with CSPG, except that entactin showed particular affinity for EL. These results offer a more detailed perspective on previous survey observations of CSPG, HSPG, and entactin in the rat lung, and describe the immunoreactivity of CS for the first time.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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