NADPH oxidase-independent formation of extracellular DNA traps by basophils
| Autor: | Kazushige Obata-Ninomiya, Alexander Eggel, Dagmar Simon, Shida Yousefi, Hans-Uwe Simon, Andrew F. Walls, Hajime Karasuyama, Md. Mahbubul Morshed, Ruslan Hlushchuk, Valentin Djonov, Thomas Kaufmann |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Extracellular Traps Immunology chemical and pharmacologic phenomena Complement factor I Basophil Proinflammatory cytokine Mice parasitic diseases Extracellular medicine Animals Humans Immunology and Allergy Receptor NADPH oxidase Innate immune system biology Receptors IgE Complement C5 NADPH Oxidases hemic and immune systems DNA Immunity Innate Basophils 3. Good health Cell biology medicine.anatomical_structure biology.protein Female Interleukin-3 Tryptases |
| Zdroj: | Journal of Immunology Journal of immunology (Baltimore Md. : 1950) |
| DOI: | 10.4049/jimmunol.1303418 |
| Popis: | Basophils are primarily associated with a proinflammatory and immunoregulatory role in allergic diseases and parasitic infections. Recent studies have shown that basophils can also bind various bacteria both in the presence and the absence of opsonizing Abs. In this report, we show that both human and mouse basophils are able to produce mitochondrial reactive oxygen species and to form extracellular DNA traps upon IL-3 priming and subsequent activation of the complement factor 5 a receptor or FcεRI. Such basophil extracellular traps (BETs) contain mitochondrial, but not nuclear DNA, as well as the granule proteins basogranulin and mouse mast cell protease 8. BET formation occurs despite the absence of any functional NADPH oxidase in basophils. BETs can be found in both human and mouse inflamed tissues, suggesting that they also play a role under in vivo inflammatory conditions. Taken together, these findings suggest that basophils exert direct innate immune effector functions in the extracellular space. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|