Cell-cell signaling in co-cultures of macrophages and fibroblasts
| Autor: | Dolly J. Holt, David W. Grainger, Lisa M. Chamberlain |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Lipopolysaccharides
Male Cell signaling Cell type Cellular differentiation Biophysics Bioengineering Cell Count Cell Communication Biology Article Cell Line Biomaterials Paracrine signalling Mice Cell–cell interaction Cell Adhesion Animals Autocrine signalling Cell Shape Cell Proliferation Macrophages Fibroblasts Juxtacrine signalling Coculture Techniques Cell biology Mice Inbred C57BL Kinetics Mechanics of Materials Culture Media Conditioned Ceramics and Composites Cytokines Biological Assay Inflammation Mediators Cell-cell signaling Signal Transduction |
| Zdroj: | Biomaterials. 31(36) |
| ISSN: | 1878-5905 |
| Popis: | The foreign body response (FBR) comprises a general, ubiquitous host tissue-based reaction to implanted materials. In vitro cell-based models are frequently employed to study FBR mechanisms involving cell signaling responses to materials. However, these models often study only one cell type, identify only limited signals, and cannot accurately represent the complexity of in vivo inflammatory signaling. To address this issue, a cell co-culture system involving two primary effector cells of the FBR, macrophages and fibroblasts, was employed. Cell-cell signaling systems were monitored between these cell types, including long-term 1) culture of one cell type in conditioned media from the other cell type, 2) non-contacting cell co-cultures (paracrine signaling), and 3) contact co-cultures (juxtacrine signaling) of primary- and secondary-derived cells. Cell culture media and cell images were collected on Days 1, 2, 3, 7, 14, and 21 and changes in soluble protein secretion, cellular behavior, and morphology were assessed. Primary- and secondary- derived cells responded uniquely during each signaling scenario and to one another. In general higher in vitro fidelity to FBR-like responses was found in primary cell co-cultures compared to their mono-cultures and all secondary cell cultures. |
| Databáze: | OpenAIRE |
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