Development of an Enzyme-Linked Immunosorbent Assay for Rapid Detection of Dengue Virus (DENV) NS1 and Differentiation of DENV Serotypes during Early Infection
| Autor: | Yi-Chien Fang, Ren-Jy Ben, Jiunn-Jye Wey, Pei-Yun Shu, Lai Szu-Chia, Meng-Hung Tsai, Yi-Ming Hsu, Chang-Chi Lin, Po-Shiuan Hsieh, Mei-Ling Hsiao, Huang Yu-Yine, Shu-Fen Chang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Serotype medicine.drug_class viruses 030106 microbiology NS1 Enzyme-Linked Immunosorbent Assay Viral Nonstructural Proteins Biology Dengue virus Antibodies Viral Serogroup Monoclonal antibody medicine.disease_cause Sensitivity and Specificity Dengue fever 03 medical and health sciences Antigen Virology medicine Humans serotype Typing Serotyping Antigens Viral Clinical Laboratory Techniques Mortality rate typing Antibodies Monoclonal Reproducibility of Results virus diseases Outbreak Dengue Virus biochemical phenomena metabolism and nutrition medicine.disease dengue Early Diagnosis clinical diagnosis 030104 developmental biology ELISA |
| Zdroj: | Journal of Clinical Microbiology |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.00221-19 |
| Popis: | Dengue fever, caused by infections with the dengue virus (DENV), affects nearly 400 million people globally every year. Early diagnosis and management can reduce the morbidity and mortality rates of severe forms of dengue disease as well as decrease the risk of wider outbreaks. Dengue fever, caused by infections with the dengue virus (DENV), affects nearly 400 million people globally every year. Early diagnosis and management can reduce the morbidity and mortality rates of severe forms of dengue disease as well as decrease the risk of wider outbreaks. Although the early diagnosis of dengue can be achieved using a number of commercial NS1 detection kits, none of these can differentiate among the four dengue virus serotypes. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) for the detection of dengue virus (DENV) NS1 by pairing a serotype-cross-reactive monoclonal antibody (MAb) with one of four serotype-specific MAbs in order to facilitate the rapid detection of NS1 antigens and the simultaneous differentiation of DENV serotypes. A total of 146 serum samples obtained from patients suspected to be in the acute phase of DENV infection were used to evaluate the clinical application of our novel test for the detection and serotyping of DENV. The overall sensitivity rate of our test was 84.85%, and the sensitivity rates for serotyping were as follows: 88.2% (15/17) for DENV serotype 1 (DENV1), 94.7% (18/19) for DENV2, 75% (12/16) for DENV3, and 66.6% (6/9) for DENV4. Moreover, there was no cross-reactivity among serotypes, and no cross-reactivity was observed in sera from nondengue patients. Thus, our test not only enables the rapid detection of the dengue virus but also can distinguish among the specific serotypes during the early stages of infection. These results indicate that our ELISA for DENV NS1 is a convenient tool that may help elucidate the epidemiology of DENV outbreaks and facilitate the clinical management of DENV infections. |
| Databáze: | OpenAIRE |
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