Eckol Enhances Heme Oxygenase-1 Expression through Activation of Nrf2/JNK Pathway in HepG2 Cells
| Autor: | Tai-Sun Shin, Hyeung-Rak Kim, Min-Sup Lee, Na-Young Yoon, Ji-Hoe Kim, Young-Jin Jun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
MAP Kinase Signaling System
NF-E2-Related Factor 2 Active Transport Cell Nucleus Pharmaceutical Science Dioxins Antioxidants Gene Expression Regulation Enzymologic Article Nrf2 Analytical Chemistry Ecklonia stolonifera lcsh:QD241-441 chemistry.chemical_compound Phosphatidylinositol 3-Kinases lcsh:Organic chemistry Drug Discovery Humans Physical and Theoretical Chemistry Phosphorylation Protein kinase B PI3K/AKT/mTOR pathway Regulation of gene expression biology Molecular Structure Eckol Organic Chemistry Hep G2 Cells heme oxygenase biology.organism_classification Molecular biology eckol Cell biology Heme oxygenase chemistry Chemistry (miscellaneous) Molecular Medicine JNK Signal transduction Reactive Oxygen Species Proto-Oncogene Proteins c-akt Heme Oxygenase-1 |
| Zdroj: | Molecules, Vol 19, Iss 10, Pp 15638-15652 (2014) Molecules Volume 19 Issue 10 Pages 15638-15652 |
| ISSN: | 1420-3049 |
| Popis: | Eckol isolated from Ecklonia stolonifera was previously reported to exhibit cytoprotective activity with its intrinsic antioxidant activity in in vitro studies. In this study, we characterized the mechanism underlying the eckol-mediated the expression of heme oxygenase-1 (HO-1). Eckol suppressed the production of intracellular reactive oxygen species and increased glutathione level in HepG2 cells. Eckol treatment enhanced the expression of HO-1 at the both level of protein and mRNA in HepG2 cells. Enhanced expression of HO-1 by eckol was presumed to be the activation of the nuclear factor erythroid-derived 2-like 2 (Nrf2) demonstrated by its nuclear translocation and increased transcriptional activity. c-Jun NH2-terminal kinases (JNKs) and PI3K/Akt contributed to Nrf2-mediated HO-1 expression. These results demonstrate that the eckol-mediated expression of HO-1 in HepG2 cells is regulated by Nrf2 activation via JNK and PI3K/Akt signaling pathways, suggesting that eckol may be used as a natural antioxidant and cytoprotective agent. |
| Databáze: | OpenAIRE |
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