Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography
| Autor: | Marco Kloos, Gabriela Nass Kovacs, Robert L. Shoeman, Marie Luise Grünbein, M. Stricker, Stefan Haacke, Ilme Schlichting, R. Bruce Doak, Jochen Reinstein, Sylvain Lecler |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Materials science Photon Scattering Physics::Optics Cell Biology Laser Biochemistry Characterization (materials science) law.invention Photoexcitation 03 medical and health sciences Crystallography law Femtosecond Molecular Biology Ultrashort pulse Excitation 030304 developmental biology Biotechnology |
| Zdroj: | Nature methods |
| ISSN: | 1548-7105 1548-7091 |
| DOI: | 10.1038/s41592-020-0847-3 |
| Popis: | Time-resolved crystallography with X-ray free-electron lasers enables structural characterization of light-induced reactions on ultrafast timescales. To be biologically and chemically relevant, such studies must be carried out in an appropriate photoexcitation regime to avoid multiphoton artifacts, a common issue in recent studies. We describe numerical and experimental approaches to determine how many photons are needed for single-photon excitation in microcrystals, taking into account losses by scattering. Theoretical and experimental guidelines to determine laser intensity for single-photon excitation in microcrystals for ultrafast pump–probe experiments by serial femtosecond crystallography. |
| Databáze: | OpenAIRE |
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