Magnetic Labeling of Activated Microglia in Experimental Gliomas
| Autor: | Claus Zimmer, Michael Synowitz, Christiane Nolte, Helmut Kettenmann, Gerrit Fleige, Florian Seeberger |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Gliosarcoma magnetic cell labeling Microglia medicine.diagnostic_test Chemistry Central nervous system microglia Magnetic resonance imaging lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease lcsh:RC254-282 In vitro law.invention medicine.anatomical_structure Confocal microscopy law In vivo Glioma glioma medicine superparamagnetic iron oxides (USPIO) MRI |
| Zdroj: | Neoplasia: An International Journal for Oncology Research, Vol 3, Iss 6, Pp 489-499 (2001) |
| ISSN: | 1476-5586 |
| DOI: | 10.1038/sj.neo.7900176 |
| Popis: | Microglia, as intrinsic immunoeffector cells of the central nervous system (CNS), play a very sensitive, crucial role in the response to almost any brain pathology where they are activated to a phagocytic state. Based on the characteristic features of activated microglia, we investigated whether these cells can be visualized with magnetic resonance imaging (MRI) using ultrasmall superparamagnetic iron oxides (USPIOs). The hypothesis of this study was that MR microglia visualization could not only reveal the extent of the tumor, but also allow for assessing the status of immunologic defense. Using USPIOs in cell culture experiments and in a rat glioma model, we showed that microglia can be labeled magnetically. Labeled microglia are detected by confocal microscopy within and around tumors in a typical border-like pattern. Quantitative in vitro studies revealed that microglia internalize amounts of USPIOs that are significantly higher than those incorporated by tumor cells and astrocytes. Labeled microglia can be detected and quantified with MRI in cell phantoms, the extent of the tumor can be seen in glioma-bearing rats in vivo. We conclude that magnetic labeling of microglia provides a potential tool for MRI of gliomas, which reflects tumor morphology precisely. Furthermore, the results suggest that MRI may yield functional data on the immunologic reaction of the CNS. |
| Databáze: | OpenAIRE |
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