Lactobacillus plantarum CIDCA 8327: An α-glucan producing-strain isolated from kefir grains
| Autor: | Alicia Prieto, Maria Fernanda Hamet, Micaela Medrano, Analia Graciela Abraham, Pablo Jose Peruzzo, M. G. Llamas, María Virginia Gangoiti, Ana Isabel Puertas, Mª Teresa Dueñas |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Eusko Jaurlaritza |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Exopolysaccharides food.ingredient Magnetic Resonance Spectroscopy Polymers and Plastics Otras Ingenierías y Tecnologías Rhamnose 030106 microbiology INGENIERÍAS Y TECNOLOGÍAS Polysaccharide Alimentos y Bebidas 03 medical and health sciences chemistry.chemical_compound food Kefir Glucosamine Skimmed milk Spectroscopy Fourier Transform Infrared Materials Chemistry Animals Food science Glucans Glucan chemistry.chemical_classification Strain (chemistry) biology Molecular Structure Organic Chemistry Polysaccharides Bacterial food and beverages Química biology.organism_classification 030104 developmental biology Milk Biochemistry chemistry Sugars Lactobacillus plantarum |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| Popis: | 30 p.-4 fig.-3 tab. Lactobacillus plantarum CIDCA 8327 is an exopolysaccharide (EPS)-producer strain isolated from kefir with promising properties for the development of functional foods. The aim of the present study was to characterize the structure of the EPS synthesized by this strain grown in skim milk or semidefined medium (SDM). Additionally, genes involved in EPS synthesis were detected by PCR. L. plantarum produces an EPS with a molecular weight of 104 Da in both media. When grown in SDM produce an heteropolysaccharide composed mainly of glucose, glucosamine and rhamnose meanwhile the EPS produced in milk was composed exclusively of glucose indicating the influence of the sugar source. FTIR spectra of this EPS showed signals attributable to an α-glucan. Both by 1H NMR and methylation analysis it was possible to determine that this polysaccharide is a branched α-(1 → 4)-d-glucan composed of 80% linear α-(1 → 4)-d-glucopyranosyl units and 19% (1 → 4)-d-glucopyranosyl units substituted at O-3 by single α-d-glucopyranosil residues. This study was supported by grants from the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), CONICET and UNLP, the Spanish Ministry of Science and Innovation (AGL2012-40084-C03), the Basque Government (no. IT866-13). |
| Databáze: | OpenAIRE |
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