Breast adipose regulation of premenopausal breast epithelial phenotype involves interleukin 10
| Autor: | Lin-Xi Li, Rosalia C. M. Simmen, Stacy A Petty, Keith G. Wolter, Iad Alhallak, Frank A. Simmen, Richard J Ward, Ana Castro Munoz |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
medicine.medical_specialty medicine.drug_class Gene Expression Adipose tissue Breast Neoplasms Context (language use) Models Biological Epithelium Article Young Adult Endocrinology Breast cancer Cyclin D1 T-Lymphocyte Subsets Internal medicine Adipocytes Humans Medicine Breast Obesity Gonadal Steroid Hormones skin and connective tissue diseases Molecular Biology Adiposity business.industry FOXP3 Middle Aged Telomere medicine.disease Immunohistochemistry Interleukin-10 Interleukin 10 Phenotype Adipose Tissue Premenopause Adipogenesis Estrogen Cytokines Female Inflammation Mediators business Biomarkers |
| Zdroj: | J Mol Endocrinol |
| ISSN: | 1479-6813 0952-5041 |
| DOI: | 10.1530/jme-21-0100 |
| Popis: | Epidemiological studies inversely associate BMI with breast cancer risk in premenopausal women, but the pathophysiological linkage remains ill-defined. Despite the documented relevance of the ‘local’ environment to breast cancer progression and the well-accepted differences in transcriptome and metabolic properties of anatomically distinct fat depots, specific breast adipose contributions to the proliferative potential of non-diseased breast glandular compartment are not fully understood. To address early breast cancer causation in the context of obesity status, we compared the cellular and molecular phenotypes of breast adipose and matched breast glandular tissue from premenopausal non-obese (mean BMI = 27 kg/m2) and obese (mean BMI = 44 kg/m2) women. Breast adipose from obese women showed higher expression levels of adipogenic, pro-inflammatory, and estrogen synthetic genes than from non-obese women. Obese breast glandular tissue displayed lower proliferation and inflammatory status and higher expression of anti-proliferative/pro-senescence biomarkers TP53 and p21 than from non-obese women. Transcript levels for T-cell receptor and co-receptors CD3 and CD4 were higher in breast adipose of obese cohorts, coincident with elevated adipose interleukin 10 (IL10) and FOXP3 gene expression. In human breast epithelial cell lines MCF10A and HMEC, recombinant human IL10 reduced cell viability and CCND1 transcript levels, increased those of TP53 and p21, and promoted (MCF10A) apoptosis. Our findings suggest that breast adipose-associated IL10 may mediate paracrine interactions between non-diseased breast adipose and breast glandular compartments and highlight how breast adipose may program the local inflammatory milieu, partly by recruiting FOXP3+ T regulatory cells, to influence premenopausal breast cancer risk. |
| Databáze: | OpenAIRE |
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