Diversity and activity of marine bacterioplankton during a diatom bloom in the North Sea assessed by total RNA and pyrotag sequencing
| Autor: | Alexander J. Mann, Hanno Teeling, Sixing Huang, Jost Waldmann, Christian Quast, Antje Wichels, Frank Oliver Glöckner, Anna Klindworth |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Proteomics
Bacterial succession Phytoplankton bloom Molecular Sequence Data Population Dynamics Aquatic Science Algal bloom 03 medical and health sciences Species Specificity Bacterioplankton bloom RNA Ribosomal 16S Gammaproteobacteria Botany Genetics 14. Life underwater Rhodobacteraceae DNA Primers Metatranscriptomics 030304 developmental biology Diatoms 0303 health sciences Bacteria Base Sequence biology Sequence Analysis RNA 030306 microbiology Gene Expression Profiling fungi Alphaproteobacteria Genetic Variation Bacterioplankton Plankton biology.organism_classification Diatom Pyrotags Metagenomics Metaproteomics North Sea Transcriptome CAZymes |
| Zdroj: | Marine Genomics |
| Popis: | A recent investigation of bacterioplankton communities in the German Bight towards the end of a diatomdominated spring phytoplankton bloom revealed pronounced successions of distinct bacterial clades. A combination of metagenomics and metaproteomics indicated that these clades had distinct substrate spectra and consumed different algal substrates. In this study we re-analyzed samples from the initial study by total community RNA (metatranscriptomics) and 16S rRNA gene amplicon sequencing. This complementary approach provided new insights into the community composition and expressed genes as well as the assessment of metabolic activity levels of distinct clades. Flavobacteria (genera Ulvibacter, Formosa, and Polaribacter), Alphaproteobacteria (SAR11 clade and Rhodobacteraceae) and Gammaproteobacteria (genus Reinekea and SAR92 clade) were the most abundant taxa. Mapping of the metatranscriptome data on assembled and taxonomically classified metagenome data of the same samples substantiated that Formosa and Polaribacter acted as major algal polymer degraders, whereas Rhodobacteraceae and Reinekea spp. exhibited less specialized substrate spectra. In addition, we found that members of the Rhodobacteraceae and SAR92 clade showed high metabolic activity levels, which suggests that these clades played a more important role during the bloom event as indicated by their in situ abundances. © 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license |
| Databáze: | OpenAIRE |
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