In Situ Measuring Partition Coefficient at Intact Nanoemulsions: A New Application of Single-Entity Electrochemistry
| Autor: | Subhashini Elangovan, Jiyeon Kim, Hiranya Madawala, Shashika Gunathilaka Sabaragamuwe |
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| Rok vydání: | 2020 |
| Předmět: |
In situ
Chemistry Surface Properties 010401 analytical chemistry Extraction (chemistry) Analytical chemistry Electrochemical Techniques 010402 general chemistry Electrochemistry 01 natural sciences Article 0104 chemical sciences Analytical Chemistry Partition coefficient Molecular dynamics Adsorption Amphiphile Molecule Aminobiphenyl Compounds Nanoparticles Emulsions Particle Size |
| Zdroj: | Anal Chem |
| ISSN: | 1520-6882 |
| Popis: | We report a new application of the single-entity electrochemistry (SEE) to in situ measure a partition coefficient at intact nanoemulsions (NEs). The partition coefficient at intact NEs is the most crucial physicochemical property to determine the uptake of delivery molecules inside NEs. It, however, has not been unequivocally elucidated by currently existing techniques based on ex situ measurements. Herein, we apply the single-entity electrochemistry (SEE) to directly and quantitatively measure the partition coefficient at NEs in situ. In this work, we use NEs featured with amphiphilic triblock copolymer (Pluronic F-127) as a model system to extract/preconcentrate 2-aminobiphenyl (2-ABP) dissolved in the water and demonstrate a new application of SEE to in situ quantitatively estimate the amounts of 2-ABP distributed into each intact NE. Our SEE measurements reveal that the partitioning is governed by extraction of 2-ABP inside NEs rather than its adsorption on the NE surface, and this extraction is remarkably efficient with up to ∼8 orders of magnitude of the preconcentration factor, thus leading to the unprecedentedly large partition coefficient of 1.9 (±1.4) × 1010. This result implies that not only the thermodynamic distribution but also the intermolecular interaction of extracted compounds inside NEs could play a significant role in the apparent partition coefficient (P = 1.9 (±1.4) × 1010). The experimentally determined partition coefficient was validated by molecular dynamics (MD) simulations with showing a stabilizing role of intermolecular interaction in the partitioned system. We further verified our methodology with other compounds exhibiting aromatic properties, e.g., ferrocenemethanol. Significantly, our new approach can be readily applicable to investigate practical NEs commercially marketed for drug, food, and cosmetics. |
| Databáze: | OpenAIRE |
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