Antimicrobial resistance profiling and molecular typing of ruminant-borne isolates of Clostridium perfringens from Xinjiang, China
| Autor: | Meng Qingling, Wang Lixia, Li Jing, Yu Weiwei, Zhang Jinsheng, Li Yan, Cai Kuojun, Ning Chengcheng, Wang Xiaoting, Peng Yelong, Ji Chunhui, Cai Xuepeng, Zhang Zaichao, Qiao Jun |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
China Clostridium perfringens Immunology Biology Molecular typing medicine.disease_cause Microbiology Antibiotic resistance Genotype Multiplex polymerase chain reaction Drug Resistance Bacterial medicine Immunology and Allergy Animals Typing Feces Antimicrobial resistance profiles Sheep Ruminant-borne isolates Ruminants QR1-502 Anti-Bacterial Agents Multiple drug resistance Clostridium Infections Multilocus sequence typing Cattle Multilocus Sequence Typing |
| Zdroj: | Journal of Global Antimicrobial Resistance, Vol 27, Iss, Pp 41-45 (2021) |
| ISSN: | 2213-7173 |
| Popis: | Objectives Clostridium perfringens (C. perfringens) can cause intestinal diseases in livestock and humans, which seriously threatens the healthy development of animal husbandry and human food safety. Here, the characteristics of antimicrobial resistance and molecular typing of ruminant-borne strains of C. perfringens in Xinjiang, China were explored and profiled. Methods A total of 307 clinical feces collected from ruminants (cattle and sheep) with diarrheal symptoms were screened for C. perfringens. The recovered isolates were characterized in respect to their antimicrobial resistance pattern and molecular typing. Results A total of 109 isolates of C. perfringens were isolated from 307 clinical feces of ruminants, most of which displayed the multidrug resistance (MDR) phenotype. Demonstration of the quinolone-resistance gene was the highest among the isolates (70.6%). The multiplex PCR typing based on toxin genes showed that type A and type D strains made up 82.6% (90/109) and 17.4% (19/109), among which, the isolates carrying β2 gene occupied 43.3% (39/90) of type A strains and 31.6% (6/19) of type D strains. These isolates were divided into 6 genotypes (I-VI) by enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) method. A total of 33 ST types (ST1-ST33) were identified by multilocus sequence typing (MLST) method. Conclusion C. perfringens isolates with multidrug resistance (MDR) were frequent and circulating in ruminants. Among them, type A-Ⅰ-ST19 was the dominant genotype of C. perfringens, displaying obvious genetic diversity. This study provided important epidemiological data for the risk assessment of food safety associated with ruminant-borne C. perfringens in Xinjiang, China. |
| Databáze: | OpenAIRE |
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