Prognostic impact of urokinase-type plasminogen activator (uPA) and its inhibitor (PAI-1) in cytosols and pellet extracts derived from 892 breast cancer patients
| Autor: | Maxime P. Look, J.G.M. Klijn, Th.J. Benraad, C.G.J. Sweep, H. A. Peters, N. Grebenschikov, J.A. Foekens, J.H. de Witte, J. J. T. M. Heuvel, Th H van Tienoven, W. L. J. Van Putten |
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| Přispěvatelé: | Medical Oncology |
| Rok vydání: | 1999 |
| Předmět: |
Adult
Cancer Research medicine.medical_specialty Steroid hormone receptor Mammary gland Breast Neoplasms Enzyme-Linked Immunosorbent Assay Biology Disease-Free Survival plasminogen activator inhibitor chemistry.chemical_compound Cytosol breast cancer Breast cancer SDG 3 - Good Health and Well-being Development of assays for prognostic factors in oncological endocrinology Internal medicine Plasminogen Activator Inhibitor 1 Biomarkers Tumor medicine Humans Clinical significance prognostic impact Aged Aged 80 and over chemistry.chemical_classification Urokinase Analysis of Variance urokinase-type plasminogen activator Regular Article Middle Aged Prognosis medicine.disease Enzyme medicine.anatomical_structure Endocrinology Oncology chemistry Plasminogen activator inhibitor-1 Ontwikkeling van meetmethodes voor prognostische factoren in de oncologische endocrinologie Female Plasminogen activator medicine.drug |
| Zdroj: | British Journal of Cancer, 79, 7/8, pp. 1190-1198 British Journal of Cancer British Journal of Cancer, 79(7/8), 1190-1198. Nature Publishing Group British Journal of Cancer, 79, 1190-1198 |
| ISSN: | 0007-0920 |
| DOI: | 10.1038/sj.bjc.6690191 |
| Popis: | To evaluate the clinical relevance of urokinase-type plasminogen activator (uPA) and its type-1 inhibitor (PAI-1) measured by a recently developed enzyme-linked immunosorbent assay (ELISA), we analysed both components in samples derived from 892 patients with primary breast cancer (median follow-up 99 months). The assays were performed in cytosolic extracts as well as in corresponding detergent extracts of pellets obtained after ultracentrifugation, which was carried out when preparing the cytosolic fractions for routine steroid hormone receptor determination. Statistically significant correlations were found between the cytosolic levels and those determined in the pellet extracts (Spearman correlation coefficient rs = 0.60, P < 0.0001 for uPA and rs = 0.65, P < 0.0001 for PAI-1). Furthermore, strong correlations were found between the levels of both uPA (rs = 0.85, P < 0.0001) and PAI-1 (rs = 0.90, P < 0.0001) in the cytosols and their levels previously measured with ELISAs based on commercial reagents. In both Cox univariate and multivariate analysis, high cytosolic levels of uPA or PAI-1 were significantly associated with increased rates of relapse and death. The levels of uPA and PAI-1 in the pellet extracts also provided prognostic information, although to a lesser extent compared with the cytosolic extracts. The prediction of prognosis on the basis of uPA and PAI-1 assessed by an alternative ELISA once again emphasizes the established prognostic role and usefulness of these parameters in selection of breast cancer patients at high or low risk of recurrence. © 1999 Cancer Research Campaign |
| Databáze: | OpenAIRE |
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