American Journal of Clinical Nutrition
| Autor: | Maria C. Gazzaneo, Samer W. El-Kadi, Marta L. Fiorotto, Scot R. Kimball, Claire Boutry, Hanh V. Nguyen, Neeraj Srivastava, Renán A. Orellana, Agus Suryawan, Teresa A. Davis |
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| Přispěvatelé: | Animal and Poultry Sciences |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
protein synthesis Anabolism Swine medicine.medical_treatment Eukaryotic Initiation Factor-2 Muscle Proteins Medicine (miscellaneous) Weight Gain Suidae Insulin Amino Acids Nutrition and Dietetics biology Chemistry Body Fluid Compartments Original Research Communications medicine.anatomical_structure Adipose Tissue Body Composition Female Signal Transduction medicine.medical_specialty growth 03 medical and health sciences Internal medicine medicine Animals Humans skeletal muscle Muscle Skeletal Protein kinase B body composition Ribosomal Protein S6 Kinases Infant Newborn Skeletal muscle Feeding Behavior biology.organism_classification infant Spine Insulin receptor Eukaryotic Initiation Factor-4E 030104 developmental biology Endocrinology Animals Newborn Protein Biosynthesis biology.protein Lean body mass Energy Intake Proto-Oncogene Proteins c-akt Hormone |
| Zdroj: | The American Journal of Clinical Nutrition. 108:830-841 |
| ISSN: | 0002-9165 |
| DOI: | 10.1093/ajcn/nqy133 |
| Popis: | Background: Orogastric tube feeding is indicated in neonates with an impaired ability to ingest food normally and can be administered with an intermittent bolus or continuous feeding schedule. Objectives: The objectives were to 1) compare the long-term effect of continuous with intermittent feeding on growth using the newborn pig as a model, 2) determine whether feeding frequency alters lean tissue and fat mass gain, and 3) identify the signaling mechanisms by which protein deposition is controlled in skeletal muscle in response to feeding frequency. Design: Neonatal pigs were fed the same amount of a balanced formula by orogastric tube either as an intermittent bolus meal every 4 h (INT) or as a continuous infusion (CON). Body composition was assessed at the start and end of the study by dual-energy X-ray absorptiometry, and hormone and substrate profiles, muscle mass, protein synthesis, and indexes of nutrient and insulin signaling were measured after 21 d. Results: Body weight, lean mass, spine length, and skeletal muscle mass were greater in the INT group than in the CON group. Skeletal muscle fractional protein synthesis rates were greater in the INT group after a meal than in the CON group and were associated with higher circulating branched-chain amino acid and insulin concentrations. Skeletal muscle protein kinase B (PKB) and ribosomal protein S6 kinase phosphorylation and eukaryotic initiation factor (eIF) 4E-eIF4G complex formation were higher, whereas eIF2 alpha phosphorylation was lower in the INT group than in the CON group, indicating enhanced activation of insulin and amino acid signaling to translation initiation. Conclusions: These results suggest that when neonates are fed the same amounts of nutrients as intermittent meals rather than continuously there is greater lean growth. This response can be ascribed, in part, to the pulsatile pattern of amino acids, insulin, or both induced by INT, which enables the responsiveness of anabolic pathways to feeding to be sustained chronically in skeletal muscle. National Institute of Arthritis and Musculoskeletal and Skin Diseases grants [AR044474, AR46308]; National Institute of Child Health and Human Development grants [HD072891, HD085573]; USDA National Institute of Agriculture grant [2013-67015-20438]; USDA-Agricultural Research ServiceUnited States Department of Agriculture (USDA) [6250-510000-055] Supported by National Institute of Arthritis and Musculoskeletal and Skin Diseases grants AR044474 (TAD) and AR46308 (MLF), National Institute of Child Health and Human Development grants HD072891 (TAD) and HD085573 (TAD), USDA National Institute of Agriculture grant 2013-67015-20438 (TAD), and by the USDA-Agricultural Research Service under cooperative agreement 6250-510000-055 (TAD). Public domain – authored by a U.S. government employee |
| Databáze: | OpenAIRE |
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