NMR metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system
| Autor: | Susan J. Wong, Ioana Amzuta, Rafael Encarnacion, Dan David, L. Joseph Wheat, William T. Hu, Joanne M. Ondrush, Andrew M. Brunner, Caitlin D. French, Josefina Fernandez, Waleed Javaid, Gregory K. Robbins, Andrés Páez, Carolyn M. Slupsky, John F. Foley, Anne M. Neilan, Amy Pan, Darya O. Mishchuk, Naaz Fatteh, Rodney E. Willoughby |
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| Přispěvatelé: | Rupprecht, Charles E |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
RNA viruses
0301 basic medicine Central Nervous System Male Viral Diseases Magnetic Resonance Spectroscopy Physiology Encephalomyelitis RC955-962 Disease Neurodegenerative Biochemistry Nervous System 0302 clinical medicine Cerebrospinal fluid Lyme disease Drug Metabolism Central Nervous System Diseases Zoonoses Arctic medicine. Tropical medicine Medicine and Health Sciences Metabolites Child Pathology and laboratory medicine Cerebrospinal Fluid Medical And Health Sciences Meningoencephalitis Neurodegenerative Diseases Medical microbiology Biological Sciences Body Fluids 3. Good health Infectious Diseases medicine.anatomical_structure Neurology Child Preschool Viruses Neurological Female Metabolic Pathways Anatomy Pathogens Public aspects of medicine RA1-1270 Infection West Nile virus Research Article Neglected Tropical Diseases Cell Physiology Multiple Sclerosis Adolescent Rabies Immunology Central nervous system Microbiology Autoimmune Diseases 03 medical and health sciences Young Adult Rare Diseases Clinical Research Tropical Medicine medicine Humans Metabolomics Pharmacokinetics Preschool Pharmacology Flaviviruses business.industry Multiple sclerosis Organisms Viral pathogens Neurosciences Public Health Environmental and Occupational Health Biology and Life Sciences Cell Biology Tropical Diseases medicine.disease Demyelinating Disorders Cell Metabolism Microbial pathogens Brain Disorders Vector-Borne Diseases Metabolism Emerging Infectious Diseases Good Health and Well Being 030104 developmental biology Clinical Immunology Clinical Medicine business 030217 neurology & neurosurgery |
| Zdroj: | French, Caitlin D; Willoughby, Rodney E; Pan, Amy; Wong, Susan J; Foley, John F; Wheat, L Joseph; et al.(2018). NMR metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system. PLOS NEGLECTED TROPICAL DISEASES, 12(12), e0007045. doi: 10.1371/journal.pntd.0007045. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/4563h9tv PLoS Neglected Tropical Diseases, Vol 12, Iss 12, p e0007045 (2018) PLoS neglected tropical diseases, vol 12, iss 12 PLoS Neglected Tropical Diseases |
| DOI: | 10.1371/journal.pntd.0007045. |
| Popis: | Background Myriad infectious and noninfectious causes of encephalomyelitis (EM) have similar clinical manifestations, presenting serious challenges to diagnosis and treatment. Metabolomics of cerebrospinal fluid (CSF) was explored as a method of differentiating among neurological diseases causing EM using a single CSF sample. Methodology/Principal findings 1H NMR metabolomics was applied to CSF samples from 27 patients with a laboratory-confirmed disease, including Lyme disease or West Nile Virus meningoencephalitis, multiple sclerosis, rabies, or Histoplasma meningitis, and 25 controls. Cluster analyses distinguished samples by infection status and moderately by pathogen, with shared and differentiating metabolite patterns observed among diseases. CART analysis predicted infection status with 100% sensitivity and 93% specificity. Conclusions/Significance These preliminary results suggest the potential utility of CSF metabolomics as a rapid screening test to enhance diagnostic accuracies and improve patient outcomes. Author summary Inflammation of the brain and spinal cord, known as encephalomyelitis, is a dangerous condition that can be caused by a wide range of pathogens, such as viruses and bacteria, and other medical conditions including autoimmunity or drug intoxications. Given the many possible causes, it is often difficult for clinicians treating patients with encephalomyelitis to identify the underlying cause, which in turn determines the appropriate treatment. Infections and other diseases causing neurological inflammation work by distinct biological mechanisms and, consequently, can cause unique biochemical changes that can be observed in cerebrospinal fluid of affected individuals. The researchers used a metabolomics technique to measure a range of small molecules in cerebrospinal fluid and examine biochemical differences in patients with encephalomyelitis caused by Lyme disease, West Nile Virus, multiple sclerosis, rabies, or fungal infection. The researchers found distinct differences in the biochemical profiles of patients whose encephalomyelitis was caused by infections versus patients with no infection, and also identified different patterns among the individual diseases. This study showed that metabolomics may be useful in improving diagnosis and treatment of diseases affecting the central nervous system by enhancing understanding of their unique effects on metabolism. |
| Databáze: | OpenAIRE |
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