Vaccinia virus myocarditis in mice: An electron microscopic and virus assay study
| Autor: | Joseph L. Melnick, Erwin R. Rabin, Alfred B. Jenson, C. Alan Phillips |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
Pathology
medicine.medical_specialty Necrosis Myocarditis viruses Clinical Biochemistry Electrons Vaccinia virus Viremia Biology Virus Pathology and Forensic Medicine Mice chemistry.chemical_compound Vaccinia medicine Virus maturation Animals Myocyte Molecular Biology Microscopy Research medicine.disease Virology Microscopy Electron chemistry Biological Assay medicine.symptom Generalized vaccinia |
| Zdroj: | Experimental and Molecular Pathology. 4:98-111 |
| ISSN: | 0014-4800 |
| DOI: | 10.1016/0014-4800(65)90026-2 |
| Popis: | A correlative study involving virus assay and electron microscopic examination has been conducted of vaccinia virus myocarditis in mice. Mice between 5 and 7 days of age developed generalized vaccinia viral infection 4–7 days after subcutaneous inoculation. Histologic evidence of myocarditis was seen in more than one fourth of these mice. Virus was recovered in relatively high titers from the heart (10 6 PFU per gram) during the period when myocardial injury occurred; viremia was also present, but virus titers in the blood stream remained much below those in the heart. By electron microscopy, various morphologic forms of vaccinia virus particles were observed which were considered related to the sequence of virus maturation. Virus particles appeared occasionally in endothelial and interstitial cells of the heart, but most particles were observed in the cytoplasm of myocytes where they were seen between myofibrils and adjacent to mitochondria. Evidence of cellular damage was almost always present in myocytes containing clusters of developing virus particles. Early pathologic change in myocytes consisted of dilation of the sarcoplasmic reticulum and perinuclear space. More extensive changes involved mitochondria, formation of numerous dense osmiophilic bodies, and necrosis of the cell. |
| Databáze: | OpenAIRE |
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