Circular RNA WDR77 target FGF-2 to regulate vascular smooth muscle cells proliferation and migration by sponging miR-124
| Autor: | Lianqun Cui, Hongjun Sang, Junjiang Chen, Yuqing Zhang, Jingliang Yuan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Vascular smooth muscle Biophysics Biology Fibroblast growth factor Biochemistry Muscle Smooth Vascular 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Circular RNA Cell Movement Humans Molecular Biology Cells Cultured Cell Proliferation Oligonucleotide Array Sequence Analysis Base Sequence Microarray analysis techniques Cell Biology RNA Circular Molecular biology In vitro Fold change MicroRNAs 030104 developmental biology HEK293 Cells 030220 oncology & carcinogenesis High glucose RNA Fibroblast Growth Factor 2 Transcriptome Transcription Factors |
| Zdroj: | Biochemical and biophysical research communications. 494(1-2) |
| ISSN: | 1090-2104 |
| Popis: | Increasing evidences have revealed the important role of circular RNAs (circRNAs) in cardiovascular system disease. Whereas, the expression profiles and in-depth regulation of circRNAs on vascular smooth muscle cells (VSMCs) is still undetermined. In present study, our research team performed circRNAs microarray analysis to present the circRNAs expression profiles in high glucose induced VSMCs in vitro. Results showed that total of 983 circRNAs were discovered to be differentially expressed, and of these, 458 were upregulated and 525 were downregulated. Moreover, 31 circRNAs were up-regulated and 22 circRNAs were down-regulated with 2 fold change (P 0.05). One of an up-regulated circRNA, circWDR77, was identified. In vitro cell assay, circWDR77 silencing significantly inhibited the proliferation and migration. Bioinformatics methods discovered that miR-124 and fibroblast growth factor 2 (FGF-2) were downstream targets of circWDR77. The RNA sequence complementary binding was validated by RNA immunoprecipitation (RIP) and/or luciferase reporter assay. Further function validation experiments revealed that circWDR77 regulated VSMCs proliferation and migration via targeting miR-124/FGF2. Taken together, present study firstly reveals the circRNAs expression profiles in high glucose induced VSMCs and identifies the role of circWDR77-miR-124-FGF2 regulatory pathway in VSMCs proliferation and migration, which might provide a new theoretical basis for diabetes mellitus correlated vasculopathy. |
| Databáze: | OpenAIRE |
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