Similar hemostatic responses to hypovolemia induced by hemorrhage and lower body negative pressure reveal a hyperfibrinolytic subset of non-human primates

Autor: James A. Bynum, Cassondra Bauer, Morten Zaar, Maryanne C. Herzig, Robert E. Shade, Wilfred Delacruz, Chriselda G. Fedyk, Heather F. Pidcoke, Andrew P. Cap, Bijaya K. Parida, Victor A. Convertino, Carmen Hinojosa-Laborde, Gary W. Muniz, Nicolas Prat, Robbie K. Montgomery
Rok vydání: 2020
Předmět:
Male
Physiology
Plasmin
medicine.medical_treatment
Hypovolemia
Monkeys
030204 cardiovascular system & hematology
Pathology and Laboratory Medicine
Vascular Medicine
Tissue plasminogen activator
Hemostatics
0302 clinical medicine
Baboons
Animal Cells
Blood plasma
Medicine and Health Sciences
Mammals
Hemodilution
Multidisciplinary
medicine.diagnostic_test
Fibrinolysis
Eukaryota
Hematology
Hyperfibrinolysis
Body Fluids
Blood
Vertebrates
Medicine
Anatomy
Cellular Types
medicine.symptom
Research Article
medicine.drug
Primates
Platelets
medicine.medical_specialty
Science
Hemorrhage
Blood Plasma
03 medical and health sciences
Signs and Symptoms
Diagnostic Medicine
Internal medicine
Old World monkeys
medicine
Animals
Blood Coagulation
Lower Body Negative Pressure
Hemostasis
Blood Cells
Biology and life sciences
business.industry
Organisms
030208 emergency & critical care medicine
Cell Biology
medicine.disease
Thromboelastography
Endocrinology
Amniotes
business
Plasminogen activator
Papio
Zdroj: PLoS ONE, Vol 15, Iss 6, p e0234844 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: BackgroundTo study central hypovolemia in humans, lower body negative pressure (LBNP) is a recognized alternative to blood removal (HEM). While LBNP mimics the cardiovascular responses of HEM in baboons, similarities in hemostatic responses to LBNP and HEM remain unknown in this species.MethodsThirteen anesthetized baboons were exposed to progressive hypovolemia by HEM and, four weeks later, by LBNP. Hemostatic activity was evaluated by plasma markers, thromboelastography (TEG), flow cytometry, and platelet aggregometry at baseline (BL), during and after hypovolemia.ResultsBL values were indistinguishable for most parameters although platelet count, maximal clot strength (MA), protein C, thrombin anti-thrombin complex (TAT), thrombin activatable fibrinolysis inhibitor (TAFI) activity significantly differed between HEM and LBNP. Central hypovolemia induced by either method activated coagulation; TEG R-time decreased and MA increased during and after hypovolemia compared to BL. Platelets displayed activation by flow cytometry; platelet count and functional aggregometry were unchanged. TAFI activity and protein, Factors V and VIII, vWF, Proteins C and S all demonstrated hemodilution during HEM and hemoconcentration during LBNP, whereas tissue plasminogen activator (tPA), plasmin/anti-plasmin complex, and plasminogen activator inhibitor-1 did not. Fibrinolysis (TEG LY30) was unchanged by either method; however, at BL, fibrinolysis varied greatly. Post-hoc analysis separated baboons into low-lysis (LY30 2%) whose fibrinolytic state matched at both HEM and LBNP BL. In high-lysis, BL tPA and LY30 correlated strongly (r = 0.95; PConclusionsCentral hypovolemia induced by either LBNP or HEM resulted in activation of coagulation; thus, LBNP is an adjunct to study hemorrhage-induced pro-coagulation in baboons. Furthermore, this study revealed a subset of baboons with baseline hyperfibrinolysis, which was strongly coupled to tPA and uncoupled from TAFI activity.
Databáze: OpenAIRE
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