Development of a novel multiphysical approach for the characterization of mechanical properties of musculotendinous tissues
| Autor: | Frank Lafont, Sandra Même, Redouane Ternifi, William Même, Philippe Pouletaut, John R. Hawse, Malek Kammoun, Frédéric Szeremeta, Jean-Marc Constans, Jessem Landoulsi, Sabine F. Bensamoun, Vincent Dupres, Malayannan Subramaniam |
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| Přispěvatelé: | Biomécanique et Bioingénierie (BMBI), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Laboratoire de Réactivité de Surface (LRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), CHU Amiens-Picardie, CHirurgie, IMagerie et REgénération tissulaire de l’extrémité céphalique - Caractérisation morphologique et fonctionnelle - UR UPJV 7516 (CHIMERE), Université de Picardie Jules Verne (UPJV), Department of Biochemistry and Molecular Biology, Mayo Clinic, Labex MS2TR01-DE14036, ANR-11-IDEX-0004,SUPER,Sorbonne Universités à Paris pour l'Enseignement et la Recherche(2011), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), LEGOUPIL, Laëtitia, Sorbonne Universités à Paris pour l'Enseignement et la Recherche - - SUPER2011 - ANR-11-IDEX-0004 - IDEX - VALID |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Response to therapy [SDV.BA] Life Sciences [q-bio]/Animal biology MESH: biomechanical phenomena/methods [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging [SDV]Life Sciences [q-bio] Muscle Fibers Skeletal [INFO.INFO-IM] Computer Science [cs]/Medical Imaging lcsh:Medicine Microscopy Atomic Force Tendons Mice 0302 clinical medicine [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing [SPI.MECA.BIOM] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] Ultrasound elastography lcsh:Science Mice Knockout Multidisciplinary medicine.diagnostic_test [PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Atomic force microscopy [SDV.BA]Life Sciences [q-bio]/Animal biology [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] Magnetic Resonance Imaging 3. Good health Tendon [SDV] Life Sciences [q-bio] DNA-Binding Proteins medicine.anatomical_structure Chemical agents Musculoskeletal models [PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph] Elasticity Imaging Techniques Female Elastography [PHYS.PHYS.PHYS-DATA-AN] Physics [physics]/Physics [physics]/Data Analysis Statistics and Probability [physics.data-an] [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing [PHYS.PHYS.PHYS-DATA-AN]Physics [physics]/Physics [physics]/Data Analysis Statistics and Probability [physics.data-an] Sarcomeres Materials science [INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing [PHYS.NEXP] Physics [physics]/Nuclear Experiment [nucl-ex] [SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] [SDV.BC]Life Sciences [q-bio]/Cellular Biology [PHYS.NEXP]Physics [physics]/Nuclear Experiment [nucl-ex] Achilles Tendon Article MESH: biomedical engineering/methods 03 medical and health sciences Elastic Modulus medicine [INFO.INFO-IM]Computer Science [cs]/Medical Imaging Animals [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics Muscle Skeletal [SDV.BC] Life Sciences [q-bio]/Cellular Biology [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing MESH: image processing computer assisted/statistics and numerical data [SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph] [SPI.ACOU] Engineering Sciences [physics]/Acoustics [physics.class-ph] [PHYS.PHYS.PHYS-MED-PH] Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph] lcsh:R Disease progression Skeletal muscle Microscopy Electron 030104 developmental biology [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging lcsh:Q Biological physics 030217 neurology & neurosurgery Biomedical engineering Transcription Factors |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2019, 9 (1), ⟨10.1038/s41598-019-44053-1⟩ Scientific Reports, 2019, 9 (1), ⟨10.1038/s41598-019-44053-1⟩ Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-44053-1⟩ |
| Popis: | At present, there is a lack of well-validated protocols that allow for the analysis of the mechanical properties of muscle and tendon tissues. Further, there are no reports regarding characterization of mouse skeletal muscle and tendon mechanical properties in vivo using elastography thereby limiting the ability to monitor changes in these tissues during disease progression or response to therapy. Therefore, we sought to develop novel protocols for the characterization of mechanical properties in musculotendinous tissues using atomic force microscopy (AFM) and ultrasound elastography. Given that TIEG1 knockout (KO) mice exhibit well characterized defects in the mechanical properties of skeletal muscle and tendon tissue, we have chosen to use this model system in the present study. Using TIEG1 knockout and wild-type mice, we have devised an AFM protocol that does not rely on the use of glue or chemical agents for muscle and tendon fiber immobilization during acquisition of transversal cartographies of elasticity and topography. Additionally, since AFM cannot be employed on live animals, we have also developed an ultrasound elastography protocol using a new linear transducer, SLH20-6 (resolution: 38 µm, footprint: 2.38 cm), to characterize the musculotendinous system in vivo. This protocol allows for the identification of changes in muscle and tendon elasticities. Such innovative technological approaches have no equivalent to date, promise to accelerate our understanding of musculotendinous mechanical properties and have numerous research and clinical applications. |
| Databáze: | OpenAIRE |
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