Micro-CT analysis of alveolar bone healing using a rat experimental model of critical-size defects
| Autor: | Shinji Kamakura, Junko Hatakeyama, M. Onodera, H. Ebina, Takahiro Honma, Yasuyuki Sasano, Hidetoshi Shimauchi |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Molar Pathology medicine.medical_specialty Bone Regeneration Time Factors X-ray microtomography Periodontal Ligament Alveolar Bone Loss Bone Matrix Dentistry Bone healing Osteocytes Collagen Type I Imaging Three-Dimensional Alveolar Process medicine Animals Periodontal fiber Mandibular Diseases Rats Wistar Tooth Root Bone regeneration General Dentistry In Situ Hybridization Dental alveolus Wound Healing Osteoblasts Chemistry business.industry Alveolar process X-Ray Microtomography Rats Disease Models Animal medicine.anatomical_structure Otorhinolaryngology Connective Tissue business Type I collagen |
| Zdroj: | Oral Diseases. 15:273-280 |
| ISSN: | 1601-0825 1354-523X |
| DOI: | 10.1111/j.1601-0825.2009.01522.x |
| Popis: | Objective: This study was designed to establish a rat model of a critical size alveolar bone defect. Materials and methods: Standardized buccal or mesiobuccal alveolar bone defects were made around the right first mandibular molar of 12-week-old rats, and the left was used as a control. Alveolar bone healing was examined quantitatively by three-dimensional micro-computed tomographic imaging. Bone matrix production of osteoblasts and osteocytes during repair of alveolar bone defects was examined with in situ hybridization for type I collagen. Results: Buccal defects were repaired significantly and the volume decreased by 88.3% in week 24, whereas mesiobuccal defects were repaired little. Osteoblasts and osteocytes expressed type I collagen in both defects in week 3 but showed little expression by week 6 and thereafter, leaving the mesiobuccal defects largely unrepaired. Conclusion: The mesiobuccal defect is a critical-size defect that is not ultimately repaired with bone. It may be an appropriate experimental model for investigating the effectiveness of bone regenerative agents in human alveolar bone loss. |
| Databáze: | OpenAIRE |
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